Functional Glycomics

The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology'„¢ series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls.Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.

Preface	p. v
Contributors	p. ix
Functional Glycomics and Glycobiology: An Overview	p. 1
Historical Overview of Glycoanalysis	p. 9
Quantitative Glycomics	p. 31
Human Gangliosides and Bacterial Lipo-oligosaccharides in the Development of Autoimmune Neuropathies	p. 51
Biotinylated Multivalent Glycoconjugates for Surface Coating	p. 67
Profiling LPS Glycoforms of Non-typeable Haemophilus influenzae by Multiple-Stage Tandem Mass Spectrometry	p. 79
In Vitro Reconstitution of Escherichia colt O86 O Antigen Repeating Unit	p. 93
Glycoprotein Characterization	p. 111
N-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Performance Liquid Chromatography and Electrospray Tandem Mass Spectrometry	p. 133
Microarray-Based Study of Carbohydrate-Protein Binding	p. 145
The Application of NMR Spectroscopy to Functional Giycomics	p. 155
Metabolomics in Giycomics	p. 175
Characterization of Lipid-Linked Oligosaccharides by Mass Spectrometry	p. 187
A Glycomics Approach to the Discovery of Potential Cancer Biomarkers	p. 199
Extraction of Chondroitin/Dermatan Sulfate Glycosaminoglycarts from Connective Tissue for Mass Spectrometric Analysis	p. 215
N-Linked Protein Glycosylation in a Bacterial System	p. 227
Characterization of Polysaccharides Using Mass Spectrometry for Bacterial Serotyping	p. 245
Is Permethylation Strategy Always Applicable to Protein N-Glycosylation Study?: A Case Study on the O-Acetylation of Sialic Acid in Fish Serum Glycans	p. 259
Bioinformatics in Giycomics: Glycan Characterization with Mass Spectrometric Data Using SimGlycanÖ	p. 269
Subject Index	p. 283
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