G Protein-Coupled Receptor--Protein

This exciting volume elucidates how GPCRs organize signal transduction and control intracellular activities. Chapters written by the recognized expert in each respective topic offer state-of-the-art updates on the principles and technology, as well as stepwise protocols for methods currently applied to the analysis of protein-receptor interactions.

SUSAN R. GEORGE, PhD, MD, FRCP, FACP, is Professor in the Departments of Medicine and Pharmacology at the University of Toronto and a Section Head of Molecular Pharmacology at the Centre for Addiction and Mental Health, Toronto.

BRIAN F. O'DOWD, PhD, is Professor in the Department of Pharmacology at the University of Toronto and a Section Head of Molecular Pharmacology at the Centre for Addiction and Mental Health, Toronto.

Foreword

ix

Robert J. Lefkowitz

Series Preface

xi

David R. Sibley

Preface

xiii

Susan R. George

Brian F. O'Dowd

Contributors

xv

Purification of the G Protein-Coupled Receptor Rhodopsin for Structural Studies

1

(18)

David Salom

Ning Li

Li Zhu

Izabela Sokal

Krzysztof Palczewski

Cooperativity in Ligand Binding to Deduce Receptor-Receptor Interaction

19

(18)

Meritxell Vivo

Philip G. Strange

Analysis of Receptor-Receptor Interactions Using Time-Resolved Fluorescence Resonance Energy Transfer

37

(16)

Douglas Ramsay

Graeme Milligan

Real-Time Imaging of GPCR-Mediated Arrestin Translocation as a Strategy to Evaluate Receptor-Protein Interactions

53

(28)

Robert H. Oakley

Larry S. Barak

Marc G. Caron

Localization of G Protein-Coupled Receptors and Interacting Proteins in Membrane Microdomains

81

(12)

K. Saidas Nair

Vijaya Narayanan

Vladlen Z. Slepak

Identification of Peptide Ligand-Binding Domains Within G Protein-Coupled Receptors Using Photoaffinity Labeling

93

(24)

Maoqing Dong

Laurence J. Miller

Analysis in Real-Time of the Assembly and Disassembly of Soluble G Protein-Coupled-Receptor-G Protein Complexes

117

(26)

Sean M. Biggs

Peter I. Simons

Anna Waller

Terry Foutz

Tione Buranda

Eric R. Prossnitz

Larry A. Sklar

Approaches to Studying Membrane Protein Oligomerization: Use of Cysteine Crosslinking Strategies to Analyze Receptor-Receptor Interactions

143

(22)

Jonathan A. Javitch

Wen Guo

Marisa E. Ring

Co-Immunoprecipitation as a Strategy to Evaluate Receptor-Receptor or Receptor-Protein Interactions

165

(14)

Randy A. Hall

Use of MALDI-TOF and Mass Spectrometry in the Evaluation of Receptor-Protein Interactions

179

(22)

John R. Raymond

Henry Ayiku

Justin Turner

Andrew Gelasco

John M. Arthur

Use of the Yeast Two-Hybrid System to Detect Receptor-Protein Interactions

201

(20)

Ridwan Lin

Nadine Kabbani

Alicia Binda

Victor Canfield

Robert Levenson

GST Fusion Protein and Pull-Down Strategies to Evaluate Receptor-Receptor or Receptor-Protein Interactions

221

(12)

Frank J.S. Lee

Fang Liu

G Protein Fusion to Monitor Integral Membrane Protein-Protein Interactions

233

(24)

Henrik Dohlman

Kathleen Ehrhard

Enzyme-Linked Immunosorbent Assays as Tools in Studying Interactions of G Protein-Coupled Receptors

257

(22)

Torsten Schoneberg

Katrin Sangkuhl

Holger Rompler

Angela Schulz

Electrophysiological Characterization of GPCR and Ligand-Gated Channel Cross-Talk

279

(28)

Tak Pan Wong

Xian-Ming Yu

Yu Tian Wang

BRET as a Strategy to Evaluate Receptor-Receptor or Receptor-Protein Interactions

307

(14)

Karin A. Eidne

Kevin D.G. Pfleger

Index

321

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G Protein-Coupled Receptor--Protein Interactions

Summary

Author Biography

Table of Contents

Supplemental Materials