Gene Quantification

The Immune Response Corporation, Carlsbad, CA. Surveys gene quantification methods and technologies research. Special attention is given to a distinction between relative quantification, for biological applications; and accurate quantification, for medical/clinical applications. Halftone graphs and tables. For researchers.

LIST OF CONTRIBUTORS

viii

(3)

FOREWORD

xi

Edwin M. Southern

Key Issues, Challenges and Future Opportunities in Gene Quantification

1

(18)

Francois Ferre

PART I: METHODS/TECHNOLOGY ISSUES

19

(234)

A. GENE QUANTITATION BASED ON PCR AMPLIFICATION

19

(150)

Present and Future Detection Formats for PCR Quantitation of Nucleic Acids

19

(12)

Shirley Kwok

John Sninsky

Determination of Target Copy Number of Quantitative Standards Used in PCR Based Diagnostic Assays

31

(14)

Zhuang Wang

Joanne Spadoro

Quantification of Specific Nucleic Acids, Regulated RNA Processing and Genomic Polymorphisms Using Reversed-Phase HPLC

45

(34)

A. Hayward-Lester

B.S. Chilton

P.A. Underhill

P.J. Oefner

P.A. Doris

Capillary Electrophoresis for Quantitative Genetic Analysis

79

(18)

Jill M. Kolesar

John G. Kuhn

Quantitative PCR Technology

97

(14)

Lincoln McBride

Mark Oldham

Statistical Estimations of PCR Amplification Rates

111

(18)

Jean Peccoud

Christine Jacob

Fluorescence Monitoring of Rapid Cycle PCR for Quantification

129

(16)

Carl Wittwer

Kirk Ririe

Randy Rasmussen

Kinetic Elisa-PCR: A Versatile Quantitative PCR Method

145

(24)

Olivier Lantz

Elizabeth Bonney

Scott Umlauf

Yassine Taoufik

B. GENE QUANTITATION BASED ON OTHER TARGET AMPLIFICATION SYSTEMS

169

(36)

Quantitation of RNA by NASBA: Applications and Issues for HIV-1 and AIDS

169

(20)

Joseph Romano

Paul van de Wiel

Stuart Geiger

Application of Transcription-Mediated Amplification to Quantification of Gene Sequences

189

(16)

Frank Gonzales

Sherrol H. McDonough

C. GENE QUANTITATION BASED ON SIGNAL AMPLIFICATION

205

(48)

Branched DNA (bDNA) Technology for Direct Quantification of Nucleic Acids: Design and Performance

205

(20)

Mark L. Collins

Peter J. Dailey

Lu-Ping Shen

Mickey S. Urdea

Linda J. Wuestehube

Janice A. Kolberg

Hybrid Capture^(TM) -- A Sensitive Signal Amplified Test for the Detection and Quantitation of Human Viral and Bacterial Pathogens

225

(28)

Attila T. Lorincz

Mariana G. Meijide

James G. Lazar

Abel De La Rosa

PART II: APPLICATIONS

253

(116)

Quantification of Gene Expression by Competitive RT-PCR: The hCG(Beta)/LH(Beta) Gene Cluster

253

(12)

Vladimir Lazar

Ivan Bieche

Michel Bahuau

Yres Giovangrandi

Dominique Bellet

Michel Vidaud

Quantitative Detection of Mycoplasma DNA Using Competitive PCR

265

(12)

Maninder K. Sidhu

Mei-June Liao

Abbas Rashidbaigi

The Detection and Quantification of bcr-abl in Chronic Myeloid Leukemia Following Marrow Transplantation

277

(18)

Jerald Radich

Competitive RT-PCR Analysis of Brain Gene Expression During Inflammation and Disease

295

(18)

Douglas L. Feinstein

Elena Galea

Development and Application of Real-Time Quantitative PCR

313

(14)

P. Mickey Williams

Todd Giles

Ayly Tucker

Jane Winer

Chris Heid

Branched DNA (bDNA) Technology for Direct Quantification of Nucleic Acids: Research and Clinical Applications

327

(16)

Janice A. Kolberg

Marcia E. Lewis

Quantification of Plasmid DNA Expression in Vivo

343

(26)

Marston Manthorpe

Jukka Hartikka

H. Lee Vahlsing

Michael Sawdey

Index

369

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Summary

Table of Contents

Supplemental Materials

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Gene Quantification

Summary

Table of Contents

Supplemental Materials

Digital License