Gene Therapy Protocols

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ISBN: 9781588299031 | 1588299031
Cover: Hardcover
Copyright: 4/9/2008

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In this book internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors and Design and Characterization of Gene Transfer Vectors. Readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for applications in stem cell biology, cancer research and infectious disease.

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Preface	p. v
Contributors	p. ix
Preparation and Quantification of Pseudotyped Retroviral Vector	p. 1
Production of Retroviral Vectors for Clinical Use	p. 17
Methods for the Production of Helper-Dependent Adenoviral Vectors	p. 33
Methods for the Production of First Generation Adenoviral Vectors	p. 55
Large-Scale Production of Recombinant Adeno-Associated Viral Vectors	p. 79
Construction and Production of Recombinant Herpes Simplex Virus Vectors	p. 97
Plasmid-Based Gene Transfer in Mouse Skeletal Muscle by Electroporation	p. 115
Chitosan Nanoparticle-Mediated Gene Transfer	p. 127
PEG-PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues	p. 141
Non-Viral Gene Delivery with Cationic Liposome-DNA Complexes	p. 159
Applications of Lentiviral Vectors in Noninvasive Molecular Imaging	p. 177
Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia A	p. 203
Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells	p. 213
Assessment of CFTR Function after Gene Transfer In Vitro and In Vivo	p. 229
Oncolytic Adenoviruses for Cancer Gene Therapy	p. 243
Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Therapy	p. 259
Prevention of Type 1 Diabetes in NOD Mice by Genetic Engineering of Hematopoietic Stem Cells	p. 277
Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells	p. 287
Nanoparticle-Mediated Gene Delivery to the Lung	p. 301
Retroviral-Mediated Gene Therapy for the Differentiation of Primary Cells into a Mineralizing Osteoblastic Phenotype	p. 333
In Vivo siRNA Delivery to the Mouse Hypothalamus Shows a Role of the Co-Chaperone XAP2 in Regulating TRH Transcription	p. 355
Efficient Retroviral Gene Transfer to Epidermal Stem Cells	p. 367
Index	p. 381
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Gene Therapy Protocols

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