Handbook of Fluorescence Spectroscopy and Imaging From Ensemble to Single Molecules

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Markus Sauer studied chemistry in Karlsruhe, Saarbrcken, and Heidelberg, where he gained his PhD in physical chemistry in 1995. After a short-term visit to LBNL, Berkeley, in the group led by Professor Shimon Weiss, he was honored with the Bio-Future award in 1998 to perform independent research on single-molecule handling, detection, and identification. In 2002 he gained his lecturing qualification at the Institute of Physical Chemistry in Heidelberg, 2003 he has accepted a Professor position for Applied Laser Physics and Laser Spectroscopy at Bielefeld University, and since 2009 he is Professor for Biotechnology and Biophysics at Wrzburg University. Professor Sauer's research interests cover super-resolution imaging methods, the development of new electron transfer sensors and probes as well as new single molecule sensitive fluorescence spectroscopic techniques. Johan Hofkens received his MSc. (1988) and Ph.D. degree (1993) in Chemistry from the University of Leuven (K.U.Leuven). After postdoctoral research with Prof. Masuhara at Osaka University and Prof. Barbara at the University of Minneapolis, he rejoined the K.U.Leuven supervising the Single Molecule Unit in the group of Prof. De Schryver. In 2005 he was appointed Research Professor at the K.U.Leuven and recently he was promoted to full professor. His research interests are fast spectroscopy, (single molecule) fluorescence microscopy and nanoscopy and the application of these techniques in material science and biosciences. Jrg Enderlein studied physics at Mechnikov University in Odessa, Ukraine, and received his PhD from Humboldt University in Berlin, Germany, in 1991. Thereafter, he worked at PicoQuant until 1996, when he joined the group under Richard A. Keller in Los Alamos, USA as a visiting scientist for one year, before becoming an assistant professor at the University of Regensburg, Germany. Since 2001 he had been a Heisenberg Fellow of the German Research Council (DFG) and established his research group at the Institute for Biological Information Processing 1 at the Forschungszentrum Jlich. After an appointment as professor for Biophysical Chemistry at Eberhard-Karls-University Tbingen, he became professor for biophysics at the Georg-August-University in Gttingen in 2007. Professor Enderlein's research focuses on the development of new single-molecule spectroscopic and imaging techniques for biophysics applications.