High-Field EPR Spectroscopy on Proteins and

"This book offers a comprehensive overview of experimental techniques in, and paradigmatic examples of, the application of high-field EPR spectroscopy in biology and chemistry. It focuses on the use of the technique in conjunction with site-specific mutation strategies and advanced quantum-chemical computation methods to reveal protein structure and dynamics. This yields new insights into biological processes at the atomic and molecular level." "The theoretical and instrumental background of high-field EPR is described and examples of paradigmatic protein systems, such as photosynthesis, are discussed in the light of recent investigations. Aspects of structure-dynamics-function relations that are revealed by studying site-specific mutants are highlighted, thereby combining high-field EPR with genetic engineering techniques. The information obtained complements that obtained from protein crystallography, solid-state NMR, infrared and optical spectroscopy."--BOOK JACKET.

Summary	p. xiii
Acknowledgements	p. xv
Introduction
Why EPR at High Magnetic Fields?	p. 3
NMR versus EPR	p. 7
From Basic to Advanced Multifrequency EPR, a Chronological Account	p. 9
References	p. 17
Principles and Illustrative Examples of High-Field/High-Frequency EPR
Spin Hamiltonians and EPR Experiments at High Magnetic Fields	p. 23
Organic Radicals and Low-Spin Transition-Metal Ions (S=1/2)	p. 23
Triplet States and High-Spin Transition-Metal Ions (S>1/2)	p. 33
High-Field EPR, ENDOR, TRIPLE, ESEEM, PELDOR and RIDME	p. 35
ENDOR and TRIPLE Hyperfine Spectroscopy	p. 35
Liquid-Solution Steady-State ENDOR and TRIPLE	p. 37
Liquid-Solution Transient EPR and ENDOR in Photochemistry	p. 53
Solid-State Pulse ENDOR and TRIPLE	p. 73
ESEEM Hyperfine Spectroscopy	p. 79
Electron-Electron Dipolar Spectroscopy	p. 93
PELDOR	p. 97
RIDME	p. 100
High-Field RIDME and PELDOR on Nitroxide Radical Pairs	p. 102
References	p. 113
Instrumentation
Experimental Techniques	p. 125
Continuous-Wave EPR (cw EPR)	p. 125
Time-Resolved EPR (TREPR)	p. 126
Pulse EPR	p. 129
Historical Overview of High-Field/High-Frequency EPR Spectrometers	p. 131
First Generation	p. 133
Second Generation	p. 134
Technical Aspects of High-Field/High-Frequency EPR	p. 137
Sensitivity Considerations	p. 137
Detection Schemes	p. 138
Microwave Sources	p. 143
Resonators	p. 145
Single-Mode Cavities	p. 146
Fabry-Perot Resonators	p. 148
Loop-Gap Resonators	p. 152
Dielectric Resonators	p. 153
Nonresonant Systems	p. 154
Microwave Transmission Lines	p. 156
Magnet Systems	p. 158
High-Field Multipurpose Spectrometers Built at FU Berlin	p. 160
The 95-GHz Spectrometer	p. 160
Microwave Bridge Design	p. 162
Magnet and Cryostat	p. 163
Probeheads	p. 164
EPR and ENDOR Performance	p. 169
Field-Jump PELDOR	p. 170
Dual-Frequency PELDOR	p. 171
The 360-GHz Spectrometer	p. 172
Quasioptical Microwave Propagation	p. 172
Microwave-Bridge Design	p. 174
Quasioptical Components	p. 176
Induction-Mode Operation	p. 176
Magnet and Cryostat	p. 178
Probeheads	p. 178
ENDOR	p. 179
Transient EPR Bridge with Reference Arm	p. 180
Pulsed Orotron Source	p. 182
References	p. 186
Computational Methods for Data Interpretation
References	p. 203
Applications of High-Field EPR on Selected Proteins and their Model Systems
Introduction	p. 206
Nonoxygenic Photosynthesis	p. 213
Multifrequency EPR on Bacterial Photosynthetic Reaction Centers (RCs)	p. 217
X-Band EPR and ENDOR Experiments	p. 219
95-GHz EPR on Primary Donor Cations P.+ in Single-Crystal RCs	p. 223
360-GHz EPR on Primary Donor Cations P.+ in Mutant RCs	p. 228
Results of g-tensor Computations of P.+	p. 232
95-GHz EPR and ENDOR on the Acceptors QA.- and QB.-	p. 234
95-GHz ESE-Detected EPR on the Spin-Correlated Radical Pair P.+QA.-	p. 248
95-GHz RIDME and PELDOR on the Spin-Correlated Radical Pair P.+QA.-	p. 250
Multifrequency EPR on Primary Donor Triplet States in RCs	p. 268
Multifrequency EPR on Bacteriorhodopsin (BR)	p. 272
Site-Directed Nitroxide Spin Labelling	p. 274
Hydrophobic Barrier of the BR Proton-Transfer Channel	p. 275
Modelling of Solute-Solvent Interactions	p. 278
Conformational changes during the BR photocycle	p. 281
Oxygenic Photosynthesis	p. 283
Multifrequency EPR on Doublet States in Photosystem I (PS I)	p. 284
Multifrequency EPR on Doublet States in Photosystem II (PS II)	p. 288
Photoinduced Electron Transfer in Biomimetic Donor-Acceptor Model Systems	p. 290
Introduction	p. 290
Covalently Linked Porphyrin-Quinone Dyad and Triad Model Systems	p. 294
Base-Paired Porphyrin-Quinone and Porphyrin-Dinitrobenzene Complexes	p. 308
DNA Repair Photolyases	p. 314
Introduction	p. 314
High-Field EPR and ENDOR Experiments	p. 319
Colicin A Bacterial Toxin	p. 323
Introduction	p. 323
Models of Transmembrane Ion-Channel Formation	p. 326
95-GHz EPR Studies of Membrane Insertion	p. 327
References	p. 330
Conclusions and Perspectives
References	p. 364
Subject Index	p. 366
Table of Contents provided by Ingram. All Rights Reserved.

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High-Field EPR Spectroscopy on Proteins and Their Model Systems

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