Human Cell Culture Protocols

Expert scientific and clinical investigators present proven human cell culture techniques applicable to tissue samples taken from a wide variety of organs, particularly those prone to pathological change. They describe in clear, step-by-step instructions the special requirements for successfully culturing such human cells as T-cells, trophoblast cells, renal cells, natural killer cells, endothelial cells, neurons, epithelial cells, pituitary cells, and more. The protocols eliminate much of the chore of adapting techniques initially developed for animal cell culture systems or the time spent in hunting down potentially useful techniques buried in the details of research papers, or even in books largely devoted to animal cell culture. They allow researchers to use successfully high quality in vitro cultures as models to explore the disease process.

Establishment and Maintenance of Normal Human Keratinocyte Cultures. Cultivation of Normal Human Epidermal Melanocytes. Cultivation of Keratinocytes from the Outer Root Sheath of Human Hair Follicles. Isolation and Short-Term Culture of Human Epidermal Langerhans' Cells. Growth and Differentiation of Human Adipose Stromal Cells in Culture. Human Fetal Brain Cell Culture. Culture of Human Normal Brain and Malignant Brain Tumors for Cellular Molecular and Pharmacological Studies. Cell Culture of Human Brain Tumors on Extracellular Matrices: Methodology and Biological Applications. Isolation and Culture of Human Umbilical Vein Endothelial Cells. Human Thymic Epithelial Cell Cultures. Generation and Cloning of Antigen-Specific Human T-Cells. Protection of Purified Human Hematopoietic Progenitor Cells by Interleukin-1b or Tumor Necrosis Factor-a. Human Mononuclear Phagocytes in Tissue Culture. Purification of Peripheral Blood Natural Killer Cells. Cystic Fibrosis Airway Epithelial Cell Culture. Gene Transfer into Cystic Fibrosis Airway Epithelial Cells. Genetic Analysis of Cystic Fibrosis Airway Epithelial Cells. Human Tracheal Gland Cells in Primary Culture. Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation. Isolation and Culture of Bone-Forming Cells (Osteoblasts) from Human Bone. The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures. In Vitro Cellular Systems for Studying Osteoclast Functionand Differentiation: Primary OC and the FLG 29.1 Model. Isolation Purification and Growth of Human Skeletal Muscle Cells. Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta. Human Myometrial Smooth Muscle Cells and Cervical Fibroblasts in Culture: A Comparative Study. Primary Culture of Human Antral Endocrine Cells. Primary Cell Culture of Human Enteric Neurons: Submucosal Plexus. Isolation and Culture of Human Hepatocytes. Culture of Human Pancreatic Islet Cells. Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules. Glomerular Epithelial and Mesangial Cell Culture and Characterization. Enzymatic Isolation and Serum-Free Culture of Human Renal Cells: Retaining Properties of Proximal Tubule Cells. Culture of Human Renal Medullary Interstitial Cells. Culture Technique of Human Pituitary Adenoma Cells. Preparation of Human Trophoblast Cells for Culture. Superfused Microcarrier Cultures of BeWo Choriocarcinoma Cells: A Dynamic Model for Studies of Human Trophoblast Function. Corneal Organ Culture. Keratocyte Cell Culture. Conjunctiva: Organ and Cell Culture. Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium. Demonstration of Mycoplasma Contamination in Cell Cultures by a Mycoplasma Group-Specific Polymerase Chain Reaction. Index.