Introduction to Proteomics

Proteomics: Introduction to Methods and Applications is the ideal introduction to the field of proteomics, giving readers the skills and knowledge needed to study proteins, their structure, and their function in cells, tissues, and organisms. Written by experienced researchers, the text not only covers all the basic concepts, techniques, and applications, it also offers readers insights and practical tips based on the authors' many years of hands-on work in proteomics research. Moreover, the text has been carefully edited to ensure a thorough, consistent, and highly readable approach throughout all the chapters.

Agnieszka Kraj, PHD, is an Associate Professor of the Neurobiochemistry Department in the Faculty of Chemistry at Jagiellonian University, Poland.

Jerzy Silberring, PHD, is the Head of the Neurobiochemistry Department in the Faculty of Chemistry as well as the former deputy head of the Regional Laboratory for Physicochemical Analyses at Jagiellonian University, Poland. Dr. Silberring is coeditor of Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research and Mass Spectrometry: Instrumentation, Interpretation, and Applications (both from Wiley).

Contributors	p. xi
Preface	p. xv
Introduction to Proteomics and Strategy for Protein Identification	p. 1
Sample Preparation	p. 7
Chromatographic Separation Methods	p. 13
Liquid Chromatography Linked to Mass Spectrometry	p. 15
Multidimensional Liquid Chromatography Combined with Mass Spectrometry	p. 23
Electrophoretic Separation Methods	p. 31
One-Dimensional Gel Electrophoresis	p. 33
Two-Dimensional Gel Electrophoresis	p. 43
Capillary Electrophoresis	p. 55
Protein Blotting	p. 65
Introduction to Protein and Peptide Mass Spectrometry	p. 77
Electrospray Ionization	p. 79
Matrix-Assisted Laser Desorption/Ionization	p. 89
Imaging Mass Spectrometry	p. 101
Fragmentation and Sequencing in Proteomics	p. 113
Protein Identification by Mass Spectrometry	p. 123
Chemical Derivatization for Sequence Analysis	p. 143
Protein Modifications	p. 153
Microfluidics	p. 181
Proteomics	p. 187
Quantitative Proteomics	p. 189
Structural Proteomics	p. 199
Functional Proteomics	p. 217
Clinical Proteomics	p. 233
Bioinformatics	p. 247
Laboratory Exercises	p. 275
Data Tables	p. 293
Internet Resources	p. 299
Index	p. 305
Table of Contents provided by Ingram. All Rights Reserved.

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Introduction to Proteomics

Summary

Author Biography

Table of Contents

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Introduction to Proteomics

Summary

Author Biography

Table of Contents

Supplemental Materials

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