PART ONE Microscopic Techniques 1. Bright-Field Light Microscopy and Microscopic Measurement of Organisms 2. The Hanging Drop Slide and Bacterial Motility 3. Dark-Field Light Microscopy 4. Phase-Contrast Microscopy PART TWO Bacterial Cell Biology 5. Aseptic Technique 6. Negative Staining 7. Simple Staining 8. Gram Stain 9. Acid-Fast Staining Procedure 10. Endospore Staining 11. Capsule Staining 12. Flagella Staining PART THREE Basic Culture Techniques 13. Preparation of Microbiology Media and Equipment 14. The Spread-Plate Technique 15. The Streak-Plate Technique 16. The Pour-Plate Technique 17. Cultivation of Anaerobic Bacteria 18. Determination of Bacterial Numbers PART FOUR Microbial Biochemistry 19. Carbohydrates I: Fermentation and ß-Galactosidase Activity 20. Carbohydrates II: Triple Sugar Iron Agar Test 21. Carbohydrates III: Starch Hydrolysis 22. Lipid Hydrolysis 23. Proteins I: The IMViC Tests 24. Proteins II: Gelatin and Casein Hydrolysis 25. Proteins III: Catalase Activity 26. Proteins IV: Oxidase Test 27. Proteins V: Urease Activity 28. Proteins VI: Lysine and Ornithine Decarboxylase Tests 29. Proteins VII: Phenylalanine Deamination 30. Proteins VIII: Dissimilatory Nitrate Reduction PART FIVE Environmental Factors Affecting Growth of Microorganisms 31. Temperature 32. pH 33. Osmotic Pressure 34. The Effects of Chemical Agents on Bacteria I: Disinfectants and Other Antimicrobial Products 35. The Effects of Chemical Agents on Bacteria II: Antibiotics 36. Bacterial Growth Curve PART SIX Environmental and Food Microbiolog 37. Monitoring Water for Coliforms 38. Enumeration of Soil Microorganisms 39. Winogradsky Columns 40. Bioluminescence 41. Plate Counts and Quality Assessment of Milk PART SEVEN Medical Microbiology 42. Staphylococci 43. Pneumococcus 44. Streptococci 45. Neisseriae 46. Normal Human Flora PART EIGHT Eukaryotic Microbiology 47. Fungi I: Yeasts (Ascomycota) 48. Fungi II: Zygomycota (Rhizopus), Ascomycota (Penicillium), and Basidiomycota (Agaricus)
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