Laboratory Exercises in Microbiology

• Part One- Microscopic Techniques
• 1. Bright-Field Light Microscopy and Microscopic Measurement of Organisms
• 2. The Hanging Drop Slide and Bacterial Motility
• 3. Dark-Field Light Microscopy
• 4. Phase-Contrast Microscopy
• Part Two- Bacterial Cell Biology
• 5. Aseptic Technique
• 6. Negative Staining
• 7. Simple Staining
• 8. Gram Stain
• 9. Acid-Fast Staining Procedure
• 10. Endospore Staining
• 11. Capsule Staining
• 12. Flagella Staining
• Part Three- Basic Culture Techniques
• 13. Preparation of Microbiology Media and Equipment
• 14. The Spread-Plate Technique
• 15. The Streak-Plate Technique
• 16. The Pour-Plate Technique
• 17. Cultivation of Anaerobic Bacteria
• 18. Determination of Bacterial Numbers
• Part Four- Microbial Biochemistry
• 19. Carbohydrates I: Fermentation and -Galactosidase Activity
• 20. Carbohydrates II: Triple Sugar Iron Agar Test
• 21. Carbohydrates III: Starch Hydrolysis
• 22. Lipid Hydrolysis
• 23. Proteins I: The IMViC Tests
• 24. Proteins II: Gelatin and Casein Hydrolysis
• 25. Proteins III: Catalase Activity
• 26. Proteins IV: Oxidase Test
• 27. Proteins V: Urease Activity
• 28. Proteins VI: Lysine and Ornithine Decarboxylase Tests
• 29. Proteins VII: Phenylalanine Deamination
• 30. Proteins VIII: Dissimilatory Nitrate Reduction
• Part Five- Environmental Factors A?ecting Growth of Microorganisms
• 31. Temperature
• 32. pH
• 33. Osmotic Pressure
• 34. The E?ects of Chemical Agents on Bacteria I: Disinfectants and Other Antimicrobial Products
• 35. The E?ects of Chemical Agents on Bacteria II: Antibiotics
• 36. Bacterial Growth Curve
• Part Six- Environmental and Food Microbiology
• 37. Monitoring Water for Coliforms
• 38. Enumeration of Soil Microorganisms
• 39. Winogradsky Columns
• 40. Bioluminescence
• 41. Plate Counts and Quality Assessment of Milk
• Part Seven- Medical Microbiology
• 42. Staphylococci
• 43. Pneumococcus
• 44. Streptococci
• 45. Neisseriae
• 46. Normal Human Flora
• Part Eight- Eukaryotic Microbiology
• 47. Fungi I: Yeasts (Ascomycota)
• 48. Fungi II: Zygomycota (Rhizopus), Ascomycota, (Penicillium), and Basidiomycota (Agaricus)

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