A Laboratory Guide to the Mammalian Embryo

, by Gardner, David K.; Lane, Michelle; Watson, Andrew J.

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ISBN: 9780195142266 | 0195142268
Cover: Paperback
Copyright: 1/8/2004

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This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. Until now, there has never been such a comprehensive compendium, though there have been more focused books of protocol, such as Manipulating the MouseEmbryo, from Cold Spring Harbor. This book is intended to appeal to all constituencies, from basic experimental science to clinical and animal science applications.

Contributors

Abbreviations

xiii

1. Follicle Development in Vitro

(21)

Norah Spears, Evelyn E. Telfer, and Alison A. Murray

2. Preparation of Gametes, in Vitro Maturation, in Vitro Fertilization, and Embryo Recovery and Transfer

(17)

Michelle Lane and David K. Gardner

3. Culture of the Mammalian Preimplantation Embryo

(21)

David K. Gardner and Michelle Lane

4. Assessment of Preimplantation Embryo Development and Viability

(14)

Denny Sakkas, Michelle Lane, and David K. Gardner

5. Micromanipulation of Gametes

(23)

Gianpiero D. Palermo, Queenie V. Neri, Takumi Takeuchi, Lucinda L. Veeck, and Zev Rosenwaks

6. Micromanipulation: Biopsy

(26)

Joyce C. Harper and Alpesh Doshi

7. Analysis of Intracellular Ions in Embryos: pH and Calcium

125

(14)

Michelle Lane, Jay M. Baltz, and David K. Gardner

8. Assessment of Nutrient Uptake, Metabolite Production, and Enzyme Activity

139

(15)

David K. Gardner and Michelle Lane

9. Metabolic Pathway Activity

154

(11)

Don Rieger

10. Confocal Imaging of Structural Molecules in Mammalian Gametes

165

(19)

Calvin Simerly, Ricardo Moreno, João Ramalho-Santos, Laura Hewitson, and Gerald Schatten

11. Fluorescent in situ Hybridisation for Detection of Aneuploidy in Single Human Blastomeres for Preimplantation Genetic Diagnosis

184

(9)

Leeanda Wilton

12. Assessment of Ploidy, Telomere Length, and Telomerase Activity in Oocytes and Embryos

193

(24)

W. Allan King, Dean H. Betts, Wafa Bureau, and Parvathi K. Basrur

13. Microscale RNA Isolation from Mammalian Embryos

217

(8)

Christine Wrenzycki, Andrew J. Watson, and Michele Calder

14. Relative mRNA Transcript Abundance in Early Embryos by Reverse Transcription-Polymerase Chain Reaction

225

(12)

Paul A. De Sousa, and Andrew J. Watson

15. Characterization of Novel Genes during Early Development by Application of Differential Display RT-PCR

237

(10)

David R. Natale and Andrew J. Watson

16. Two-Dimensional Protein Gel Database Analysis of Embryos, Oocytes, and Oocyte-Associated Granulosa Cells

247

(11)

Keith E. Latham, Gula Nourjanova, Karen Wigglesworth, and John J. Eppig

17. Antisense Disruption of Gene Expression in the Preimplantation Embryo

258

(12)

Lisa C. Barcroft, D. Holstead Jones-Taggart, and Andrew J. Watson

18. Gene Subtraction and Analysis

270

(9)

Marc-André Sirard and Claude Robert

19. Analysis of Apoptosis in the Preimplantation Embryo

279

(19)

Daniel R. Brison, Anthony D. Metcalfe, Debra J. Bloor, Helen R. Hunter, Gerard Brady, and Susan J. Kimber

20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo

298

(15)

D. Page Baluch, Christine M. Pauken, and David G. Capco

21. Human Embryonic Stem Cells

313

(21)

Susan E. Lazendorf, Catherine A. Boyd, and Diane L. Wright

22. Spermatogonial Transplantation

334

(18)

Makoto C. Nagano

23. Somatic Cell Nuclear Transfer

352

(23)

Paul De Sousa, William Ritchie, Andras Dinnyes, Tim King, Lesley Paterson, and Ian Wilmut

24. Cryobiology: Slow Freezing Vitrification of Embryos

375

(16)

Tetsunori Mukaida and Magosaburo Kasai

Index

391

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A Laboratory Guide to the Mammalian Embryo

Summary

Table of Contents

Supplemental Materials