Lymphocytes A Practical Approach

Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.

Charles R. M. Bangham: Department of Immunology, Imperial College School of Medicine at St Mary's, Norfolk Place, London, UK. Judy M. Bastin: Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK. Simon C. Biddolph: Department of Paediatric Pathology, Womens' Centre, John Radcliffe Hospital, Headington, Oxford, UK. Marion H. Brown: MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, South Parks Road, Oxford, UK. Michael Browning: Department of Microbiology and Immunology, University of Leicester, Medical Sciences Building, University Road, Leicester, UK. Helmut Fickenscher: Institut fur Klinische und Molekulare Virologie, Friedrich-Alexander-Universitat Erlangen-Nurnberg,, Germany. Y. Furukawa: Department of Immunology, Imperial College School of Medicine at St Mary's, Norfolk Place, London, UK. Kevin C. Gatter: University Department of Cellular Sciences, Level 4, John Radcliffe Hospital, Headington, Oxford, UK. Andrew Carmichael: Department of Medicine, Level 5, Addenbrooke's Hospital, Cambridge, UK. P. Gorak-Stolinska: Department of Immunology, GKT School of Medicine and Dentistry, London, UK. Pete Krausa: Molecular Genetics Division, Forensic Analytical, Hayward, CA, USA. Deirdre E. J. Mcloughlin: Department of Anatomy, University of Birmingham Medical School, UK. Edgar Meinl: Max Planck-Institut fur Neurobiologie, Martinsried, Germany. Kingston H. G. Mills: Infection and Immunity Group, Department of Biology, National University of Ireland, Maynooth, County Kildare Grahams. Ogg: Molecular Human Immunology Unit, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK John J. T. Owen: Department of Anatomy, University of Birmingham Medical School, Edgbaston, Birmingham, B15 2TT, UK. Magdalena Plebanski: The Austin Research Institute, Victoria, Australia. Sarah L. Rowland-Jones: Molecular Human Immunology Unit, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK. Antony Symons: The R. W. Johnson Pharmaceutical Research Institute, San Diego, CA, USA. Rusung Tan: Department of Pathology and Laboratory Medicine, BC's Childrens' Hospital, Vancouver, BC, Canada. M. Vukmanovic-Stejic: Department of Immunology, GKT School of Medicine and Dentistry, London, UK. Anna Vyakarnam: Department of Immunology, GKT School of Medicine and Dentistry, London, UK. B. Vyas: Department of Immunology, GKT School of Medicine and Dentistry, London, UK. D. Wallace: Edward Jenner Institute for Vaccine Studies, Compton, Berkshire, UK.