The Metabolic Pathway Engineering Handbook:

This second volume of the Metabolic Engineering Handbook delves into evolutionary tools as well as. gene expression tools for metabolic pathway engineering. It covers applications of emerging technologies including recent research genome-wide technologies, DNA and phenotypic microarrays, and proteomics. tools for experimentally determining flux through pathways. This volume also looks at emerging applications for producing fine chemicals, drugs, and alternative fuels.

Christina D. Smolke is regarded as one of the most brilliant new brilliant new minds in biomedical engineering. She demonstrated the power and opportunities awaiting discovery through metabolic engineering when she developed a novel way to churn out large quantities of drugs from genetically modified brewer's yeast. And now with The Metabolic Pathway Engineering Handbook, she provides us with a reference guide to the future of biomedical engineering. Bringing together pioneering scientists from dozens of disciplines, this exciting work offers us a complete record of accomplishment in metabolic pathway engineering and lays the groundwork for those seeking to make their own contributions.

Introduction	p. ix
Editor	p. xv
Contributors	p. xvii
Evolutionary Tools in Metabolic Engineering
Evolutionary Engineering of Industrially Important Microbial Phenotypes	p. 1
Improving Protein Functions by Directed Evolution	p. 2
Engineering DNA and RNA Regulatory Regions through Random Mutagenesis and Screening	p. 3
Evolving Pathways and Genomes for the Production of Natural and Novel Compounds	p. 4
Models Predicting Optimized Strategies for Protein Evolution	p. 5
Gene Expression Tools for Metabolic Pathway Engineering
Low-Copy Number Plasmids as Artificial Chromosomes	p. 6
Chromosomal Engineering Strategies	p. 7
Regulating Gene Expression through Engineered RNA Technologies	p. 8
Tools Designed to Regulate Translational Efficiency	p. 9
Metabolic Engineering of the Secretory Processing Pathway in Eukaryotes	p. 10
Engineering Multifunctional Enzyme Systems for Optimized Metabolite Transfer between Sequential Conversion Steps	p. 11
Practical Pathway Engineering-Demonstration in Integrating Tools	p. 12
Application of Emerging Technologies to Metabolic Engineering
Genome-Wide Technologies: DNA Microarrays, Phenotypic Microarrays, and Proteomics	p. 13
Monitoring and Measuring the Metabolome	p. 14
Future Prospects in Metabolic Engineering
Systems Biology, Genome-Scale Models, and Metabolic Engineering	p. 15
Cell-Free Systems for Metabolic Engineering	p. 16
In Silico Models for Metabolic Systems Engineering	p. 17
Tools for Experimentally Determining Flux through Pathways
GC-MS for Metabolic Flux Analysis	p. 18
Tools for Measuring Intermediate and Product Formation	p. 19
Use of AEX-HPLC-ESI-MS for 13C-Labeling Based Metabolic Flux Analysis in Saccharomyces cerevisiae and Penicillium chrysogenum	p. 20
Future Applications of Metabolic Engineering
Energy and Cofactor Issues in Fermentation and Oxyfunctionalization Processes	p. 21
Microbial Biosynthesis of Fine Chemicals: An Emerging Technology	p. 22
Applications of Metabolic Engineering for Natural Drug Discovery	p. 23
Metabolic Engineering for Alternative Fuels	p. 24
Index	p. I
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The Metabolic Pathway Engineering Handbook: Tools and Applications

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