Methods in Nano Cell Biology

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ISBN: 9781597492706 | 1597492701
Cover: Hardcover
Copyright: 12/1/2008

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Understanding live cells at the single molecule level is the most important and single major challenge facing biology and medicine today. Nanobiology focuses on the properties and structure of complex assemblies of biomolecules-biochips and molecular motors, for example-in conjunction with distinctive surfaces, rods, dots, and materials of nanoscience. Nano Cell Biology will describe the current applications of nanobiology to the study of the structure, function, and metabolic processes of cells. Key Features *Provides historical background on this newly emerging field *Covers the latest application of new instrumentation in the field *Detailed protocols in the study of live cells at the nanometer level *Discusses future technologies and their applications in the study of living cells.

Contributors	p. xiii
Preface	p. xvii
Extracellular Dynamics at nm Resolution in Live Cells
Introduction	p. 2
Materials and Methods	p. 3
Conclusion	p. 16
References	p. 17
Intracellular Organelle Dynamics at nm Resolution
Introduction	p. 20
Materials and Methods	p. 20
Discussion	p. 24
References	p. 36
Atomic Force Microscopy as a Tool to Study the Proteasome Assemblies
Introduction	p. 40
Rationale	p. 44
Methods	p. 45
Discussion	p. 56
Summary	p. 59
References	p. 59
Biomineralization at the Nanoscale: Learning from Diatoms
Introduction to Nanotechnology	p. 62
Introduction to Diatoms	p. 65
Investigating Diatom Mesoscale Silica Formation	p. 67
Nanoscale Imaging of Living Diatoms	p. 69
Nanoscale Elasticity Measurements	p. 71
Examination of Diatom Valves	p. 75
Examination of Diatom Girdle Bands	p. 78
Examination of Diatom Setae	p. 80
Future Directions	p. 81
References	p. 83
Quantitative Phase Imaging of Nanoscale Cell Structure and Dynamics
Introduction and Rationale	p. 88
Methods	p. 88
Applications of QPI	p. 97
Summary and Outlook	p. 108
References	p. 110
Fourier Imaging Correlation Spectroscopy for Cellular Structure-Function
Introduction and Background	p. 118
Theoretical Descriptions of FICS	p. 119
FICS Optical Layout	p. 127
Examples of FICS Applications	p. 129
Conclusions	p. 136
References	p. 136
Nanobiology of the Primary Cilium-Paradigm of a Multifunctional Nanomachine Complex
Introduction	p. 140
Self-Assembly	p. 141
Ciliogenesis	p. 141
Elucidating the Functional Role of Primary Cilia	p. 151
The Medical Consequences of Aciliogenesis-A Veritable Pandora's Box	p. 153
Concluding Remarks	p. 153
References	p. 154
Assembly and Disassembly of SNAREs in Membrane Fusion
Introduction	p. 158
Materials and Methods	p. 161
Discussion	p. 164
References	p. 181
Understanding Membrane Fusion: Combining Experimental and Simulation Studies
Introduction	p. 184
Materials and Methods	p. 185
Discussion	p. 186
References	p. 197
Structure and Dynamics of Metalloproteins in Live Cells
Introduction	p. 200
Rationale	p. 201
Methods	p. 202
Materials	p. 205
Discussion	p. 207
Summary	p. 214
References	p. 214
Light-Activated Ion Channels for Remote Control of Neural Activity
Introduction	p. 218
Discussion	p. 221
Methods	p. 230
Conclusion	p. 231
References	p. 231
Molecular Modeling and Simulation Studies of Ion Channel Structures, Dynamics and Mechanisms
Introduction	p. 234
Homology-Based Structure Prediction of Transmembrane Proteins	p. 234
PB Profiles for the Energetics of an Ion in a Channel	p. 241
MD Simulations	p. 245
Free Energy Methods	p. 251
Summary	p. 259
References	p. 260
Nano-Scale Imaging and Dynamics of Amylin-Membrane Interactions and Its Implication in Type II Diabetes Mellitus
Introduction	p. 268
Materials and Methods	p. 270
Results and Discussion	p. 272
Summary	p. 282
References	p. 284
Real Time Investigation of Protein Folding, Structure, and Dynamics in Living Cells
Introduction	p. 288
Rationale	p. 294
Methods and Materials	p. 295
Summary	p. 322
References	p. 323
Structural Basis of Human High-density Lipoprotein Formation and Assembly at Sub nanometer Resolution
Introduction	p. 328
Rationale	p. 332
Methods and Materials	p. 333
Discussion	p. 354
Summary	p. 359
References	p. 360
Nano-scale Characterization of the Dynamics of the Chloroplast Toc Translocon
Introduction	p. 367
In organeller Chloroplast Import and Binding Assays	p. 370
Toc Translocon Dynamics	p. 377
Activity and Enzymology of Toc GTPases	p. 384
Molecular Modeling of Toc Proteins	p. 391
Conclusions and Perspectives	p. 392
References	p. 396
A System Biology Approach to Understand Functional Activity of Cell Communication Systems
Introduction	p. 400
Methods	p. 401
Materials	p. 404
Discussion	p. 412
References	p. 413
THz Investigations of Condensed Phase Biomolecular Systems
Introduction	p. 418
Instrumentation	p. 420
Theory	p. 422
Dipeptides Nanotubes	p. 423
References	p. 432
Combined Single-Molecule Electrical Recording and Single-Molecule Spectroscopy Studies of Ion Channel Conformational Dynamics
Introduction	p. 436
Development of Simultaneous Single-Molecule Fluorescence Spectroscopy and Patch-Clamp Electrical Recordings	p. 437
Summary and Prospects	p. 446
References	p. 449
Index	p. 453
Volumes in Series	p. 465
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Methods in Nano Cell Biology

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