Molecular Forensics

Note: Supplemental materials are not guaranteed with Rental or Used book purchases.

ISBN: 9780470024959 | 047002495X
Cover: Hardcover
Copyright: 5/21/2007

Rent

(Recommended)

$150.46

Term

Due

Price

Semester

Dec 13

$158.38

Quarter

Aug 16

$150.46

Short Term

Jul 16

$142.54

*This item is part of an exclusive publisher rental program and requires an additional convenience fee. This fee will be reflected in the shopping cart.
Buy New

Currently Available, Usually Ships in 24-48 Hours

$217.37

Molecular Forensics offers comprehensive coverage of the increasingly important role molecular analysis plays within forensic science. Starting with a broad introduction to modern forensic molecular technologies, the text covers key issues from the initial scenes of crime sampling to the use of evidential material in the prosecution of legal cases. The book also explores the questions raised by the growing debate on the applications of national DNA databases and the resulting challenges of developing, maintaining and curating such vast data structures. The broader range of applications to non-human cases is also discussed, as are the statistical pitfalls of using so called unique data such as DNA profiles and the ethical considerations of national DNA databases. This book is an invaluable reference work for students taking courses within the Forensic and Biomedical sciences. It is also useful for practitioners in the field looking for a broad overview of the subject.

Preface	p. ix
List of Contributors	p. xi
Current and Future Trends in Forensic Molecular Biology	p. 1
Introduction	p. 1
Developments within the field of forensic molecular biology	p. 2
Developments influencing law enforcement - operational impacts	p. 7
Developments influencing the justice system - socio-legal impacts	p. 10
Summary	p. 11
References	p. 12
Basic Tools and Techniques in Molecular Biology	p. 21
Introduction	p. 21
Isolation and separation of nucleic acids	p. 21
Automated analysis of nucleic acid fragments	p. 23
Molecular biology and bioinformatics	p. 24
The polymerase chain reaction (PCR)	p. 25
Applications of the PCR	p. 28
Nucleotide sequencing of DNA	p. 30
Conclusion	p. 34
References	p. 34
Automated DNA Extraction Techniques for Forensic Analysis	p. 37
Introduction	p. 37
Principal steps of DNA extraction	p. 38
DNA extraction techniques	p. 39
Modified techniques for DNA extraction from challenging forensic samples	p. 43
Automation of DNA extraction	p. 45
References	p. 56
Real-time Quantitative PCR in Forensic Science	p. 59
Introduction	p. 59
Current real-time PCR chemistries	p. 60
Human nuclear DNA quantification	p. 61
Human mitochondrial DNA quantification	p. 65
Detection and quantification of non-human species	p. 66
Concluding remarks and perspectives	p. 67
References	p. 67
Minisatellite and Microsatellite DNA Typing Analysis	p. 71
Introduction	p. 71
Minisatellites	p. 71
Microsatellites	p. 80
Acknowledgements	p. 86
References	p. 86
Application of SNPs in Forensic Casework	p. 91
Introduction	p. 91
Single nucleotide polymorphisms	p. 92
Single nucleotide polymorphism typing technology	p. 94
Single nucleotide polymorphisms for human identification	p. 95
Single nucleotide polymorphisms in mitochondrial DNA	p. 98
Forensic DNA phenotyping	p. 98
Ethical considerations of SNP genotyping	p. 100
References	p. 100
The X Chromosome in Forensic Science: Past, Present and Future	p. 103
Introduction	p. 103
History of forensic utilization of the X chromosome	p. 104
Chromosome X short tandem repeats	p. 107
Power of ChrX markers in trace analysis	p. 111
Power of ChrX markers in kinship testing	p. 111
Chromosome X marker mapping and haplotype analysis	p. 114
Chromosome X-chromosome Y homologue markers	p. 119
Chromosome X STR allele and haplotype distribution in different populations	p. 119
Ethical considerations in ChrX marker testing	p. 120
Concluding remarks	p. 121
References	p. 121
Mitochondrial Analysis in Forensic Science	p. 127
Introduction	p. 127
Mitochondrial DNA (mtDNA) biology	p. 128
Identification of individuals (mtDNA typing)	p. 132
Topics of forensic interest	p. 134
References	p. 138
Y-Chromosomal Markers in Forensic Genetics	p. 141
Introduction	p. 141
Identification of the male sex	p. 142
Identification of male lineages	p. 143
Identification of a male's paternity	p. 148
Identification of a male's geographical origin	p. 151
The future of Y-chromosomal markers in forensics	p. 155
Acknowledgements	p. 156
References	p. 157
Laser Microdissection in Forensic Science	p. 163
Introduction	p. 163
Histological, biochemical analysis	p. 166
References	p. 169
Laboratory Information Systems for Forensic Analysis of DNA Evidence	p. 171
Introduction	p. 171
The specifications of forensic genotyping assays	p. 173
Automated pipetting	p. 174
Analysis of STR data	p. 176
Bioinformatics	p. 178
Conclusion	p. 180
References	p. 181
Statistical Presentation of Forensic Data	p. 185
Introduction	p. 185
Techniques	p. 185
Laboratory issues	p. 188
Statistical analysis	p. 189
Other issues	p. 193
Special situations	p. 194
References	p. 195
Protein Profiling for Forensic and Biometric Applications	p. 197
Introduction	p. 197
Protein assays in molecular forensics: current status	p. 197
Novel technologies and the remaining challenges	p. 204
Protein markers for use in forensic and biometric applications	p. 208
References	p. 217
Application of MRS in Forensic Pathology	p. 221
Forensic, criminalistic and ethical significance of time of death	p. 221
Classical methods for the determination of PMI	p. 222
Magnetic resonance spectroscopy	p. 224
How to predict PMI based on MRS measurements	p. 229
Outlook	p. 232
References	p. 235
Index	p. 241
Table of Contents provided by Ingram. All Rights Reserved.

Please wait while the item is added to your bag...

FREE SHIPPING

Amazon no longer offers textbook rentals. We do!

Molecular Forensics

Summary

Author Biography

Table of Contents

Supplemental Materials