Molecular Genetics of Mammalian Cells

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ISBN: 9780121820527 | 0121820521
Cover: Hardcover
Copyright: 12/28/1987

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Strategies needed to isolate mutant cell lines and to use the mutants to clone and map the genes are presented in detail. This is the first book in which such methodology and molecular biology are combined. The preferred cell lines for genetic analysis are presented in the first section, followed by special techniques for isolating a wide range of mutants. The remainder of the book is devoted to genetic mapping and cloning of mutant genes. The final section presents special techniques in gene regulation.

Cell Lines Useful for Genetic Analysis
Chinese Hamster Ovary Cells
Cultured S49 Mouse T Lymphoma Cells
Liver Cell Lines
HeLa Cell Lines
Somatic Cell Genetic Analysis of Myelomas and Hybridomas
Mouse Teratocarcinoma Cells
Special Techniques for Mutant Selection
Amplification of Genes in Somatic Mammalian Cells
Replica Plating and Detection of Antigen Variants
Drug-Resistant Mutants: Selection and Dominance Analysis
Suicide Selection of Mammalian Cell Mutants
Use of Tritium-Labeled Precursors to Select Mutants
Construction of Immunotoxins Using Pseudomonas Exotoxin A
Isolation of Temperature-Sensitive Mutants
Fluorescence-Activated Cell Sorting of Hybrid and Transfected Cells
Genetic Mapping and Analysis
Isozyme Identification of Chromosomes in Interspecific Somatic Cell Hybrids
Principles of Electrofusion and Electropermeabilization
Cell Enucleation, Cybrids, Reconstituted Cells, and Nuclear Hybrids
Toxin*b1Antitoxin Selection for Isolating Heterokaryons and Cell Hybrids
Chromosome Sorting by Flow Cytometry
Methods for Chromosome Banding of Human and Experimental Tumors in Vitro
In Situ Hybridization of Metaphase and Prometaphase Chromosomes
Gene Mapping with Sorted Chromosomes
Transfer and Selective Retention of Single Specific Human Chromosomes via Microcell-Mediated Chromosome Transfer
Isolation and Detection of Mutant Genes
Strategies for Isolation of Mutant Genes
Use of in-Gel DNA Renaturation for Detection and Cloning of Amplified Genes
Detection and Characterization of Specific mRNA by Microinjection and Complementation of Mutant Cells
Use of the CAT Reporter Gene for Optimization of Gene Transfer into Eukaryotic Cells
Cosmid Vector Systems for Genomic DNA Cloning
Preparation of a Genomic Cosmid Library
Colony Screening of Genomic Cosmid Libraries
Rescue of Genes Transferred from Cosmid Libraries
Phage-Mediated Transduction of cDNA Expression Libraries into Mammalian Cells
Sib Selection
Repetitive Cloning of Mutant Genes Using Locus-Specific Sticky Ends
Strategies for Mapping and Cloning Macroregions of Mammalian Genomes
Insertional Mutagenesis to Isolate Genes
Microcloning of Mammalian Metaphase Chromosomes
Gene Regulation in Tissue Culture
Antisense RNA Inhibition of Endogenous Genes
In Situ Detection of Transcription in Transfected Cells Using Biotin-Labeled Molecular Probes
RNA Detection and Localization in Cells and Tissue Sections by in Situ Hybridization of 35S-Labeled RNA Probes
Electrophoretic Assay for DNA-Binding Proteins
Author Index
Subject Index
Table of Contents provided by Publisher. All Rights Reserved.

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Molecular Genetics of Mammalian Cells

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Table of Contents

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Molecular Genetics of Mammalian Cells

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