Mouse Models of Cancer: A Laboratory Manual

The study of animal virus pathogenesis is an important and rapidly developing area, especially since the emergence of AIDS and the consequent increase in research activity. Because this work takes place at the interface between virology and immunology, understanding viral pathogenesis requires knowledge and use of techniques drawn from several disciplines. This book provides up-to-date coverage of the way that viral tropism, viral expression, and viral infectivity are assayed. It describes the identification of antiviral cytotoxic T cells, natural killer and free antibody responses. It also covers making vaccinia/viral cDNA for immunologic target recognition, and tissue and whole animal in situ hybridization.

1. Concepts of Molecular Pathogenesis, Michael B.A. Oldstone
2. Molecular Characterization of Viral Infections In Vitro, W.Ian Lipkin and Michael B.A. Oldstone
3. Detection of Immune Complexes and Antibody Mediated Target Cell Lysis, Michael B.A. Oldstone
4. Detection, Generation, and Use of Cytotoxic T Lymphocyte (CTL) Clones, Michael B.A. Oldstone
5. Methods for Studying Murine Natural Killer Cells, Raymond M. Welsh
6. Viral-Receptor Binding Assays, Peter L. Schwimmbeck, Matthias Lohr, and Antoinette Tishon
7. Viral-Autoimmune Experimental Models, Robert S. Fujinami
8. Synthetic Peptides as Antigens, Thomas Dyrberg and Hans Kofod