Neuropharmacology Methods in Epilepsy Research

There is an estimated 2.5 million epileptics in the US & perhaps some 40 million worldwide. As research has become increasingly molecular in scope, fewer scientists are trained in the US on basic, integrated epilepsy techniques. One frustration in neuroscience today is the application of state-of-the-art molecular biology techniques to inappropriate animal models of epilepsy - frequently resulting in inconclusive results. Epilepsy research will be increasingly undertaken by scientists well-trained in reductionist methodology, but who may be unfamiliar with integrated, whole-animal techniques. This situation appears even more difficult considering there has been no updated textbook on experimental models of epilepsy over the last twenty years - until now. Neuropharmacology Methods in Epilepsy Research describes fundamental methodologies & procedures in this field, representing the only detailed text concerning experimental models of epilepsy published in the last 20 years. This guide studies the reproduction of well-characterized & readily interpretable experimental models of epilepsy to which state-of-the-art molecular biology techniques can be applied. Each chapter features: *Introduction - providing a brief background & historical account of the techniques & their use *Methodology - describing equipment, solutions, species, electrodes as well as considering variations of techniques & stimulation parameters *Interpretations - demonstrating the relevance of techniques to epilepsy as well as describing what exactly is being studied & how the data is appropriately applied to understanding epilepsy Topics include electroshock, chemoconvulsions, kindling, audiogenic seizures, focal seizures, & brain slice preparations. Discussions also include: *Recently developed seizure models, including status epilepticus & massed trial simulations *Influence of circadian & diurnal rhythms on convulsive activity *Behavior al & cognitive deficits associated with anticonvulsant drug testing *Technical approaches, i.e. slice models, microdialysis techniques, intracranial implant surgery, audiogenic seizure testing, kindling paradigms, & the rhythmic nature of seizures This unique text provides a thorough reference for the diverse methodologies within this area of neuropharmacological research - providing the basis for on-going cellular & molecular investigations as well as novel therapeutic approaches to the treatment of epilepsy.