Nucleic Acid Amplification Technologies

Univ. of Cambridge, U.K. Guide to current information on various nucleic acid amplification technologies for clinical laboratory diagnosis. For biomedical scientists. Available in hardcover edition only. 32 contributors, 21 U.S.

1 Evolution of Diagnostic Technologies

1

(10)

S. A. Morse

H. Lee

2 Enzymes Used in Nucleic Acid Amplification

11

(18)

E. H. Lee

H. Lee

3 Peptide Nucleic Acid

29

(20)

H. Orum

C. Kessler

T. Koch

4 Sample Preparation for Nucleic Acid Amplification

49

(12)

F. D. Quinn

5 Ligation-Based Nucleic Acid Probe Methods

61

(18)

J. J. Carrino

C. Chan

M. Canavaggio

H. Lee

6 PCR and Its Modifications for the Detection of Infectious Diseases

79

(22)

G. P. Martin

E. Timmers

7 QB Replicase Assays for the Clinical Detection of Infectious Agents

101

(12)

D. M. Olive

8 Application of Transcription-Mediated Amplification to Detection of Nucleic Acids from Clinically Relevant Organisms

113

(12)

S. H. McDonough

M. A. Bott

C. Giachetti

9 Strategies to Avoid Amplicon Contamination

125

(10)

K. Abravaya

H. Y. Hu

O. Khalil

10 Labels and Detection Formats in Amplification Assays

135

(16)

P. M. Jung

H. Y. Hu

O. S. Khalil

11 Automation and Use of Robotics in Nucleic Acid Amplification Assays

151

(14)

O. S. Khalil

12 Evaluation of Diagnostic Tests--Special Problems Introduced by DNA Amplification Procedures

165

(6)

J. Schachter

13 Amplification Methods for Detection of Food-Borne Pathogens

171

(12)

T. J. Barrett

P. Feng

B. Swaminathan

14 Nucleic Acid Amplification Techniques in Detection and Diagnosis of Medically Important Viral Infections

183

(18)

A. Sundsfjord

O. Olsvik

15 Nucleic Acid Amplification Assays for Sexually Transmitted Diseases

201

(32)

T. C. Quinn

16 Amplification for Detection of Mutations Imparting Drug Resistance in Mycobacteria

233

(12)

J. T. Crawford

17 Cyclic Reactions for the Synthesis of Artificial DNA

245

(12)

Y. E. Khudyakov

H. A. Fields

18 Nucleic Acid Amplification Strategies for Diagnosis of Heritable Diseases

257

(24)

M. Rabin

Index

281

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Nucleic Acid Amplification Technologies

Summary

Table of Contents

Supplemental Materials