Nucleic Acid Therapeutics in Cancer

A cutting-edge review of the important issues underlying the therapeutic use of nucleic acid-mediated gene silencing. Topics range from basic methodology and delivery to targeting and clinical targets. The authors thoroughly explain the latest developments in RNA biology, as well as the underpinnings of RNA interference, oligodeoxynucleotide delivery into cells, and strategies for targeting these molecules to accessible regions within the mRNA. They also provide some examples of how these new therapeutic compounds are being used clinically.

Preface

v

Contributors

ix

Part I. Introduction and Perspective

1 Antisense Methodology: An Assessment After 25 Years

3

(12)

Fritz Eckstein

2 Nucleic Acid Therapeutics: An Introduction

15

(12)

Alan M. Gewirtz

Part II. Basic Methodology

3 Targeted Genome Modification Via Triple Helix Formation

27

(18)

Faye A. Rogers and Peter M. Glazer

4 Therapeutic Applications of Ribozymes

45

(20)

John J. Rossi

5 Use of Catalytic DNA in Target Validation and Therapeutics

65

(16)

Lun-Quaiz Sun

6 Targeted Destruction of Small, Stable RNAs: Principles Applicable to Antisense Therapies

81

(8)

David A. Dunbar and Susan J. Baserga

7 Mechanism of Action of Antisense RNA in Eukaryotic Cells

89

(18)

Zuo Zhang and Gordon G. Carmichael

Part III. Delivery

8 The Transport of Oligonucleotides Into Cells

107

(8)

R.L. Juliano

9 Peptide-Mediated Delivery of Antisense Oligonucleotides and Related Material

115

(14)

Eric Vivès, Jean Philippe Richard, and Bernard Lebleu

10 Molecular Vectors for Gene Delivery to Cancer Cells

129

(14)

Guy Zuber, Jean-Serge Remy, Patrick Erbacher, Pascale Belguise, and Jean-Paul Behr

Part IV. Targeting

11 Considerations on the Design of Antisense Oligonucleotides

143

(12)

Rosel Kretschmer-Kazemi Far, Jens M. Warnecke, and Georg Sczakiel

12 Identification of Hybridization Accessible Sequence in Messenger RNA

155

(12)

Lida K. Gifford, Ponzy Lu, and Alan M. Gewirtz

Part V. Clinical Targets

13 Nucleic Acids As Gene Targeting Therapeutics

167

(10)

Joanna B. Opalfinska and Susan E. Shetzline

14 LY900003 (Isis 3521) and G3139 (Genasense; Oblimersen): Phosphorothioate Antisense Oligonucleotides With Pleiotropic Mechanisms of Action

177

(22)

C.A. Stein, Nathalie Dias, Luba Benimetskaya, Jan S. Jepsen, Johnathan C.H. Lai, and Anthony J. Raffo

15 Antisense Protein Kinase A-RIa Restores Normal Signal Transduction Signatures to Inhibit Tumor Growth

199

(14)

Yoon S. Cho-Chung

Index

213

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Nucleic Acid Therapeutics in Cancer

Summary

Table of Contents

Supplemental Materials

FREE SHIPPING

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Nucleic Acid Therapeutics in Cancer

Summary

Table of Contents

Supplemental Materials

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