Optical Microscopy : Emerging Methods and

Optical Microscopy: Emerging Methods and Applicationscovers recent technical advances and new approaches to monitoring and altering cell physiology, examining membrane cytoarchitecture, observing multiple cellular activities and intact organ physiology, plus confocal imaging of live cell function, lifetime imaging, and automated clinical imaging cytometry. The book provides the reader with a synopsis of the most recent technical developments in optical microscopy as applied to scientific research. Each chapter introduces new methods by describing how these overcome limitations inherent in previous techniques. Software, hardware, and other equipment concerns are covered. Additionally, the book reviews current applications in order to stimulate future developments in optical microscopy, encouraging novel uses and new technical advances

M. Peonie, New Fluorescent Probes of Cell Function
Photosensitive Caged Compounds: Design
Properties
And Biological Applications
Fluorescence Ratio Imaging: Errors and Artifacts
Sampling Characteristics of Ccd Video Cameras
Measurement and Manipulation of Oscillations in Cytoplasmic Calcium
Simultaneous Multiple Detection of Fluorescent Molecules: Rapid Kinetic Imagin of Calcium and Ph in Living Cells
Real-Time Fluorescence Imaging
Photolysis of Caged Compounds
And Whole-Cell Patch Clamping
Simultaneous Differential Interference Contrase and Quantitative Low-Light Fluorescence Video Imaging of Cell Function
Fluorescence Microscopic Imaging of Membrane Domains
Measurement of Membrance Glycoprotein Movement By Single Particle Tracking
Total Internal Reflectance Fluorescence Microscopy
Laser Scanning Confocal Microscopy of Living Cells
Intravital Microscopy
Time-Resolved Fluorescence Lifetime Imaging
Automated Fluorescence Image Cytometry as Applied to the Diagnosis and Understanding of Cervical Cancer
Chapter References

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Optical Microscopy : Emerging Methods and Applications

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