Organelle Proteomics

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ISBN: 9781588297792 | 1588297799
Cover: Paperback
Copyright: 2/1/2008

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This is the first book to examine organelle proteomics in depth.It begins by introducing the different analytical strategies developed and successfully utilized to study organelle proteomes, and detailing the use of multidimensional liquid chromatography coupled to tandem mass spectrometry for peptide sample analysis. Detailed protocols are provided and a section is devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle.

Preface	p. v
Contributors	p. xiii
Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry	p. 1
Analysis of Organelles, Vesicles, and Protein Machineries
Purification and Proteomic Analysis of Chloroplasts and their Sub-Organellar Compartments	p. 19
Proteomic Analysis of the Stacked Golgi Complex	p. 37
Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device	p. 51
Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria: Activity Measurement and Subunit Composition Analysis	p. 65
Mitochondrial Proteomics: Analysis of a Whole Mitochondrial Extract with Two-Dimensional Electrophoresis	p. 83
Purification and Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane	p. 101
Subcellular Fractionation and Proteomics of Nuclear Envelopes	p. 117
Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction	p. 139
Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis	p. 149
Purification and Proteomic Analysis of Plant Plasma Membranes	p. 161
Protocol to Enrich and Analyze Plasma Membrane Proteins from Frozen Tissues	p. 175
Proteomic Characterization of Membrane Protein Topology	p. 185
Free Flow Isoelectric Focusing: A Method for the Separation of Both Hydrophilic and Hydrophobic Proteins of Rat Liver Peroxisomes	p. 199
Use of Gas-Phase Fractionation to Increase Protein Identifications: Application to the Peroxisome	p. 217
Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins	p. 229
Affinity Purification of Soluble Lysosomal Proteins for Mass Spectrometric Identification	p. 243
Purification and Proteomic Analysis of Synaptic Vesicles	p. 259
Purification and Proteomics Analysis of Pancreatic Zymogen Granule Membranes	p. 275
Isolation and Proteomic Analysis of Chlamydomonas Centrioles	p. 289
Purification and Proteomic Analysis of 20S Proteasomes from Human Cells	p. 301
Characterization of E. coli Ribosomal Particles: Combined Analysis of Whole Proteins by Mass Spectrometry and of Proteolytic Digests by Liquid Chromatography-Tandem Mass Spectrometry	p. 321
Proteins Identified in the Organelle Sample: True Components or Contaminants?
Assessment of Organelle Purity Using Antibodies and Specific Assays: The Example of the Chloroplast Envelope	p. 345
Identifying Bona Fide Components of an Organelle by Isotope-Coded Labeling of Subcellular Fractions: An Example in Peroxisomes	p. 357
Determination of Genuine Residents of Plant Endomembrane Organelles using Isotope Tagging and Multivariate Statistics	p. 373
Quantitative Proteomic Analysis to Profile Dynamic Changes in the Spatial Distribution of Cellular Proteins	p. 389
Use of Transcriptomic Data to Support Organelle Proteomic Analysis	p. 403
Index	p. 415
Table of Contents provided by Ingram. All Rights Reserved.

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Organelle Proteomics

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