PCR Applications

PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom.

Key Concepts for PCR
Optimization of PCR: Conversations between Michael and David
The Convergence of PCR, Computers, and the Human Genome Project: Past, Present and Future
Thermostable DNA Polymerases: An Update
Musings on Microbial Genomes
Statistical Refinement of Primer Design Parameters
Multiplex PCR: Optimization Guidelines
The Use of Immobilized Mismatch Binding Protein for the Optimization of PCR Fidelity
A New Generation of PCR Instruments and Nucleic Acid Concentration Systems
Sequencing PCR Products
Recent Advances in High-Temperature Reverse Transcription and PCR
Viral Genotyping by a Quantitative Point Mutation Assay: Application to HIV-1 Drug Resistance
In Situ PCR
Quantitative PCR
Standards for PCR Assays
Rapid Thermal Cycling and PCR Kinetics
Kinetics of Competitive Reverse Transcriptase-PCR
Kinetic PCR Analysis Using a CCD Camera and without Using Oligonucleotide Probes
Quantification of Telomerase Activity Using Telomeric Repeat Amplification Protocol
Gene Discovery
Differential Display
Single-Cell cDNA Libraries
Whole Cell Assays
Screening Differentially Displayed PCR Products by Single-Strand Conformation Polymorphism Gels
Microsatellite Protocols
Real-Time Quantitative PCR: Uses in Discovery Research
Homology Cloning: A Molecular Taxonomy of the Archaea
Cloning Mammalian Homologs of Drosophila Genes
Cloning Human Homologs of Yeast Genes. Genomics and Expression Profiling
Cellular Transcriptome Analysis Using a Kinetic PCR Assay
Parallel Analysis with Biological Chips
High Density cDNA Grids for Hybridization Fingerprinting Experiments
Comparative Genomic Hybridization
Genetic Footprinting and Fucntional Maps of the Yeast Genome
Molecular Analysis of Microdissected Tissue: Laser Capture Microdissection
A Fluorescent, Multiplex Solid-Phase Mini Sequencing Method for Genotyping Cytochrome P450 Genes
The Cleavase Enzyme for Mutation and<$$$>
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