PCR Protocols in Molecular Toxicology

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:o Quantitative reverse transcriptase PCR and methods to examine gene expressiono Differential display cloningo Cloning and library screening by PCRo Genotype and polymorphism analysis of drug and toxicant metabolizing enzymeso Basic, non-PCR based molecular biology methodsPCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.