Plant–Pathogen Interactions

Plant-Pathogen Interactions: Methods and Protocols provides key methods, approaches, and strategies to dissect the plant defense response. Addressing methods to identify and characterize plant resistance genes as well as pathogen-associated molecules that trigger the plant defense response, this volume creates a better understanding of the interactions between pathogens and their hosts, which will help to develop better methods for disease control in plants and animals. Plant-Pathogen Interactions: Methods and Protocols reviews methods for engineering resistance to plant viruses, the utility of viral induced gene silencing and RNAi silencing, as well as advances in genomics and proteomics that have lead to new methods to identify genes and proteins. The protocols presented follow the successful Methods in Molecular Biology'„¢ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and the avoidance of known pitfalls.

Preface	p. v
Contributors	p. ix
The Use of Protoplasts to Study Innate Immune Responses	p. 1
Marker-Exchange Mutagenesis and Complementation Strategies for the Gram-Negative Bacteria Xanthomonas oryzae pv. oryzae	p. 11
Whole-Genome Analysis to Identify Type III-Secreted Effectors	p. 19
In planta Expression of Oomycete and Fungal Genes	p. 35
Use of Nipponbare BAC Clones for Physical Mapping of an R Gene Locus in Rice	p. 45
Agrobacterium-Mediated Transformation to Create an Insertion Library in Magnaporthe grisea	p. 57
Identification of Components in Disease-Resistance Signaling in Arabidopsis by Map-Based Cloning	p. 69
Yeast Two-Hybrid Approaches to Dissecting the Plant Defense Response	p. 79
Use of Rolling-Circle Amplification for Large-Scale Yeast Two-Hybrid Analyses	p. 85
Preparative Denaturing Isoelectric Focusing for Enhancing Sensitivity of Proteomic Studies	p. 99
Use of Massively Parallel Signature Sequencing to Study Genes Expressed During the Plant Defense Response	p. 105
Use of Microarray Analysis to Dissect the Plant Defense Response	p. 121
Use of Robust-Long Serial Analysis of Gene Expression to Identify Novel Fungal and Plant Genes Involved in Host-Pathogen Interactions	p. 131
Analysis of Gene Function in Rice Through Virus-Induced Gene Silencing	p. 145
Use of RNA Interference to Dissect Defense-Signaling Pathways in Rice	p. 161
Virus-Induced Gene Silencing in Plant Roots	p. 173
Methods for Engineering Resistance to Plant Viruses	p. 183
Development of Genetically Engineered Resistant Papaya for papaya ringspot virus in a Timely Manner: A Comprehensive and Successful Approach	p. 197
Index	p. 241
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Plant–Pathogen Interactions

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