Practical Methods for Biocatalysis and Biotransformations

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ISBN: 9780470519271 | 0470519274
Cover: Hardcover
Copyright: 12/30/2009

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Practical Methods for Biocatalysis and Biotransformations is a "how-to" guide focusing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting materials and reagents, hints, tips, and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given, and relevant references to the primary literature are cited. Practical Methods for Biocatalysis and Biotransformations is an essential text offering validated biocatalyst methods for researchers and postgraduate students in industry and academia.

Preface
Abbreviations
Perspectives of Biotransformations in Small Molecule Pharmaceutical Development
Biocatalyst Identification and Scale-Up: Molecular Biology For Chemists
Kinetic Resolutions Using Biotransformations
Stereo- and enantioselective hydrolysis of rac-2-octylsulfate using whole resting cells of Pseudomonas spp
Protease-catalyzed resolutions using the 3-(3-pyridine)propionyl anchor group: p-toluenesulfonamide
Desymmetrisation of prochiral ketones using enzymes
Enzymatic Resolution of 1-Methyl-tetrahydroisoquinoline (1-MTQ) using Candida Rugosa Lipase
Dynamic Kinetic Resolution For the Synthesis of Esters, Amides and Acids Using Lipases
Dynamic Kinetic Resolution of 1-Phenylethanol by Immobilized Lipase Coupled with in-situ Racemization over Zeolite Beta
Synthesis of the (R)-butyrate esters of secondary alcohols by dynamic kinetic resolution employing a bis(Tetrafluorosuccinato)-Bridged Ru(II) Complex
Dynamic Kinetic Resolution of 6,7-dimethoxy-1-methyl-1,2,3,4-tetrahydroisoquinoline
Dynamic Kinetic Resolution of Primary Amines with a Recyclable Palladium Nanocatalyst (Pd /AlO(OH)) for Racemization
Dynamic Kinetic Resolution of Amines Involving Biocatalysis and in situ Free-Radical-Mediated Racemization
Chemoenzymatic Dynamic Kinetic Resolution of (S)-Ibuprofen
Dynamic kinetic resolution synthesis of a fluorinated amino acid ester amide by a continuous process lipase-mediated ethanolysis of an azalactone
Enzymatic Selectivity in Synthetic Methods
Alcalase Catalyzed Syntheses of Hydrophilic Di- and Tri-peptides in Organic Solvents
Selective alkoxycarbonylation of 1a,25-dihydroxyvitamin D₃ diol precursor with Candida antarctica lipase B
The use of lipase enzymes for the synthesis of polymers and polymer intermediates
Bioconversion of t3-cyanopyridine to nicotinic acid with Gordona terrae NDB1165
Enzyme-promoted desymmetrization of prochiral dinitriles
Epoxide Hydrolase Catalyzed Synthesis of (R)-3-benzyloxy-2-methylpropane-1,2-diol
One-pot biocatalytic synthesis of methyl (S)-4-chloro-3-hydroxybutanoate and methyl (S)-4-cyano-3-hydroxybutanoate Maja Majeric
Aldolase Enzymes For Complex Synthesis
One step synthesis of L-fructose using rhamnulose-1-phosphate aldolase in borate buffer
Straightforward Fructose-1,6-Diphosphate Aldolase mediated synthesis of aminocyclitols
Synthesis of D-fagomine by aldol addition of dihydroxyacetone to N-Cbz-3-aminopropanal catalyzed byD-fructose-6-phosphate aldolase José
Chemoenzymatic Synthesis of 5-Thio-D-Xylopyranose
Enzymatic Synthesis of Glycosides and Glucuronides
Glycosynthase-assisted Oligosaccharide Synthesis
Glycosyl Azides - Novel Substrates for Enzymatic Transglycosylations
Facile synthesis of alkyl ß-D-glucopyranosides from D-glucose and the corresponding alcohols using fruit seed meals
Laccase-Mediated Oxidation of Natural Glycosides
Biocatalyzed synthesis of monoglucuronides of Hydroxytyrosol, Tyrosol, Homovanillic Alcohol, and 3-(4'-Hydroxyphenyl)propanol using liver cells microsomal fractions
Synthesis of the acyl glucuronide of mycophenolic acid
Synthesis of Cyanohydrins Using Hydroxynitrile Lyases
Synthesis of (S)-2-Hydroxy-2-methylbutyric Acid by a Chemoenzymatic Methodology
(S)-Selective cyanohydrin synthesis from aromatic ketones using hydroxynitrile lyases
Hydroxynitrile lyase catalysed synthesis of enantiopure (S)-acetophenone cyanohydrins
(R)- and (S)-Cyanohydrin synthesis from pyridine-3-carboxaldehyde using CLEA-immobilized hydroxynitrile lyases
A new (R)-Hydroxynitrile lyase from Prunus mume for Asymmetric Synthesis of Cyanohydrins
Synthesis of Chiral Sec-Alcohols by Ketone Reduction
Asymmetric synthesis (S)-bis-(trifluoromethyl)phenyl ethanol by biocatalytic reduction of bis-(trifluoromethyl)acetophenone
Enantioselective and diastereoselective enzyme-catalyzed dynamic kinetic resolution of an unsaturated ketone
Enzyme-catalyzed synthesis of a-alkyl-b-hydroxy ketones and esters by isolated ketoreductases
Asymmetric reduction of phenyl ring-containing ketones using xerogel-encapsulated W110A secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus
(R)- and (S)- Enantioselective diaryl methanol synthesis using enzymatic reduction of diaryl ketones
Highly enantioselective and efficient synthesis of methyl (R)-o-chloromandelate, key intermediate for clopidogrel, with recombinant E. coli
Reduction of Functional Groups
Reduction of Carboxylic Acids by Carboxylic Acid Reductase (Car) heterologously expressed in E. coli
Light-driven Stereoselective Biocatalytic Oxidations and Reductions
Unnatural amino acids by enzymatic transamination: synthesis of glutamic acid analogues with aspartate aminotransferase
Synthesis of L-Pipecolic Acid with D¹-Piperidine-2-carboxylate Reductase from Pseudomonas putida Hisaaki Mihara and Nobuyoshi Esaki
Synthesis of substituted derivatives of L-phenylalanine and of other non-natural L-amino acids using engineered mutants of phenylalanine dehydrogenase
Enzymatic Oxidation Chemistry
Monoamine Oxidase (MAO-N) Catalysed Reactions: Application Towards the Chemo-Enzymatic Deracemisation of the Alkaloid (±)-Crispine A
Glucose oxidase catalysed synthesis of aldonic acids
Oxidation and halo-hydroxylation of monoterpenes with chloroperoxidase from Caldariomyces fumago
Chloroperoxidase (CPO) Catalyzed Oxidation of Phenyl Methylsulfide in Ionic Liquids
Stereoselective synthesis of b-hydroxy sulfoxides catalyzed by cyclohexanone monooxygenase
Enantioselective kinetic resolution of racemic 3-phenylbutan-2-one using a Baeyer-Villiger monooxygenase
Desymmetrisation of 1-methylbicyclo[3.3.0]octane-2,8-dione by the retro-Claisenase 6-oxo camphor hydrolase
Synthesis of optically pure chiral lactones by cyclopentadecanone monooxygenase (CPDMO)-catalyzed Baeyer-Villiger oxidations
Whole Cell Oxidations and Dehalogenations
Biotransformations of naphthalene to 4-hydroxy-1-tetralone by Streptomyces griseus NRRL 8090
Hydroxylation of imidacloprid for the synthesis of olefin imidacloprid by Stenotrophomonas maltophilia CGMCC 1.1788
Biocatalytic synthesis of 6-hydroxy fluvastatin using Motierella rammaniana DSM 62752 in shake flask culture and on multi-gram scale using a Wave bioreactor
Synthesis of 1-adamantanol from adamantane through regioselective hydroxylation by Streptomyces griseoplanus cells
Enantioselective benzylic microbial hydroxylation of indan and tetralin
Stereospecific biotransformation of (R/S)-linalool by Corynespora cassiicola DSM 62475 into linalool oxides
The biocatalytic synthesis of 4-fluorocatechol from fluorobenzene
Synthesis of enantiopure (S)-styrene oxide by selective oxidation of styrene by recombinant E.coli JM101 (pSPZ10)
Biotransformation of a-bromo- and a,a'-dibromo alkanone into a-hydroxyketone and a-diketone by Spirulina platensis
Table of Contents provided by Publisher. All Rights Reserved.

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Practical Methods for Biocatalysis and Biotransformations

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Practical Methods for Biocatalysis and Biotransformations

Summary

Author Biography

Table of Contents

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