Prion Protein Protocols

While much information has been gained regarding the molecular nature of prion proteins, only recently have several research groups begun to cast light on turning the normal prion protein into its aberrant, infectious form. In this book, a team of experts provide the most up-to-date collection of current methods in this unique area of neuroscience. Comprehensive and cutting-edge, this is an ideal collection for researchers investigating the growing field of mammalian prion disease.

Preface	p. v
Contributors	p. xi
Cell Culture Models to Unravel Prion Protein Function and Aberrancies in Prion Diseases	p. 1
Investigation of PrP[superscript C] Metabolism and Function in Live Cells: Methods for Studying Individual Cells and Cell Populations	p. 21
Immunodetection of PrP[superscript Sc] Using Western and Slot Blotting Techniques	p. 35
Assaying Prions in Cell Culture: The Standard Scrapie Cell Assay (SSCA) and the Scrapie Cell Assay in End Point Format (SCEPA)	p. 49
Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes	p. 69
Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons	p. 83
Understanding the Nature of Prion Diseases Using Cell-free Assays	p. 97
Methods for Conversion of Prion Protein into Amyloid Fibrils	p. 105
Amplification of Purified Prions In Vitro	p. 117
Expression and Purification of Full-Length Recombinant PrP of High Purity	p. 131
Analysis of PrP Conformation Using Circular Dichroism	p. 145
Effect of Copper on the De Novo Generation of Prion Protein Expressed in Pichia pastoris	p. 161
Biophysical Investigations of the Prion Protein Using Electron Paramagnetic Resonance	p. 173
Molecular Diagnosis of Human Prion Disease	p. 197
Analysis of Endogenous PrP[superscript C] Processing in Neuronal and Non-neuronal Cell Lines	p. 229
Molecular Typing of PrP[superscript res] in Human Sporadic CJD Brain Tissue	p. 241
Transgenic Mouse Models of Prion Diseases	p. 249
Quantitative Bioassay of Surface-bound Prion Infectivity	p. 265
Index	p. 275
Table of Contents provided by Ingram. All Rights Reserved.

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Prion Protein Protocols

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