Protein Nmr Techniques

SmithKline Beecham Pharmaceuticals, Hertfordshire, U.K. Text on how to apply the fundamentals of NMR to the study of proteins. For chemists and biochemists. 29 contributors, 26 U.S.

Preface

v

(4)

Contributors

ix

1 Introduction to the NMR of Proteins

1

(28)

David G. Reid

Lesley K. MacLachlan

Andrew J. Edwards

Julia A. Hubbard

Patricia J. Sweeney

2 Double and Triple Resonance NMR Methods for Protein Assignment

29

(24)

Brain Whitehead

C. Jeremy Craven

Jonathan P. Waltho

3 Protein Chemical Shifts

53

(18)

Michael P. Williamson

Tetsuo Asakura

4 Data Processing in Multidimensional NMR

71

(86)

Istvan Pelczer

Brian G. Carter

5 Calculating Protein Structures from NMR Data

157

(38)

Peter Guntert

6 Studies of Protein-Ligand Interactions by NMR

195

(38)

David J. Craik

Jacqueline A. Wilce

7 NMR of Paramagnetic Proteins

233

(38)

Michael J. Osborne

David Crowe

Sharon L. Davy

Colin Macdonald

Geoffrey R. Moore

8 NMR Studies of Peptides and Proteins Associated with Membranes

271

(28)

R. Bryan Klassen

Stanley J. Opella

9 Metal NMR for the Study of Metalloproteins

299

(26)

Torbjorn Drakenberg

Charlotta Johansson

Sture Forsen

10 Production and Characterization of Recombinant Proteins for NMR Structural Studies

325

(12)

Danuta E. Mossakowska

Richard A. G. Smith

11 A Case History: NMR Studies of the Structure of a Small Protein, w-Conotoxin MVIIA

337

(26)

Lesley K. MacLachlan

David A. Middleton

Andrew J. Edwards

David G. Reid

12 Spectrometer Calibration and Experimental Setup: Basic Principles and Procedures

363

(48)

Ian M. Brereton

Index

411

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Protein Nmr Techniques

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