Real-Time PCR

Real-time PCR (RT-PCR) technology is highly flexible and many alternative instruments and fluorescent probe systems have been developed recently. The decreased hands-on time, increased reliability and improved quantitative accuracy of RT-PCR methods have contributed to the adoption of RT-PCR for a wide range of new applications.This essential manual presents a comprehensive guide to the most up-to-date technologies and applications as well as providing an overview of the theory of this increasingly important technique. Renowned experts in the field describe and discuss the latest PCR platforms, fluorescent chemistries, validation software, data analysis, and internal and external controls. This timely and authoritative volume also discusses a wide range of RT-PCR applications including: clinical diagnostics, biodefense, RNA expression studies, validation of array data, mutation detection, food authenticity and legislation, NASBA, molecular halotyping, and much more.An essential book for all laboratories using PCR.

List of contributors	p. v
Preface	p. ix
An Introduction to Real-Time PCR	p. 1
An Overview of PCR Platforms	p. 7
Homogeneous Fluorescent Chemistries for Real-Time PCR	p. 23
Reference Gene Validation Software for Improved Normalization	p. 47
Data Analysis Software	p. 65
Performing Real-time PCR	p. 85
Internal and External Controls for Reagent Validation	p. 95
Introduction to the Applications of Real-Time PCR	p. 105
Analysis of mRNA Expression by Real-time PCR	p. 111
Validation of Array Data	p. 137
Mutation Detection by Real-Time PCR	p. 149
Real-Time NASBA	p. 163
Applications in Clinical Microbiology	p. 177
Diagnosis of Invasive Fungal Infections	p. 221
Biodefence	p. 235
Real-time PCR: Application to Food Authenticity and Legislation	p. 253
Molecular Haplotyping by Real-time PCR	p. 269
Index	p. 279
Plates	p. A-1
Table of Contents provided by Ingram. All Rights Reserved.

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