Recombinant DNA Methodology II

The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. * Methods for: * DNA isolation and cloning * Synthesizing complementary DNA (cDNA) * Cleaving and manipulating DNA * Selecting useful reporter genes * Constructing vectors for cloning genes * Constructing expression vectors * Site-directed mutagenesis and gene disruption * Identifying and mapping genes * Transforming animal and plant cells * Sequencing DNA * Amplifying and manipulating DNA and PCR * Detecting DNA - protein interaction

Isolation, Synthesis, and Detection of DNA and RNA: J. Eberwine, C. Spencer, K. Miyashiro, S. Mackler, and R. Finnell, Complementary DNA Synthesis in Situ : Methods and Applications. S. Beck, Nonradioactive Detection of DNA Using Dioxetane Chemiluminescence. H.C. Birnboim, Extraction of High Molecular Weight RNA and DNA from Cultured Mammalian Cells. G. Levesque, A.D. Bharucha, and M.R. Ven Murthy, Biochemical Manipulations of Minute Quantities of mRNAs and cDNAs Immobilized on Cellulose Paper Discs. J. Soh and S. Pestka, Hybrid Selection of mRNA with Biotinylated DNA. Enzymes and Methods for Cleaving and Manipulating DNA: A.S. Bhagwat, Restriction Enzymes: Properties and Use. C.W. Fuller, Modified T7 DNA Polymerase for DNA Sequencing. Reporter Genes: D.E. Hruby and E.M. Wilson, Use of Fluorescent Chloramphenicol Derivative as a Substrate for Chloramphenicol Acetyltransferase Assays. S. De La Luna and J.Ortin, pac Gene as Efficient Dominant Marker and Reporter Gene in Mammalian Cells. K.R. Luehrsen, J.R. de Wet, and V. Walbot, Transient Expression Analysis in Plants Using Firefly Luciferase Reporter Gene. K. D'Halluin, M. De Block, J. Denecke, J. Janssens, J. Leemans, A. Reynaerts, and J. Botterman, The bar Gene as Selectable and Screenable Marker in Plant Engineering. Vectors for Cloning and Mapping Vectors: M.A. Alting-Mees, J.A. Sorge, and J.M. Short, pBluescriptII: Multifunctional Cloning and Mapping Vectors. J.M. Short and J.A. Sorge, In Vivo Excision Properties of Bacteriophage lambda ZAP Expression Vectors. A.D. Zelenetz, Construction of Complex Directional Complementary DNA Libraries in Sfi I. G.A. Evans, K. Snider, and G.G. Hermanson, Use of Cosmids and Arrayed Clone Libraries for Genome Analysis. K.D. Tartof, Cloning Vectors and Techniques for Exonuclease-Hybridization Restriction Mapping. Vectors for Expressing Cloned Genes: G. Sczakiel and K. Maeda, Vector pPLEX for Expression of Nonfusion Polypeptides in Escherichia coli. A. Lieber, V. Sandig, W. Sommer, S. Bahring, and M. Strauss, Stable High-Level Gene Expression in Mammalian Cells by T7 Phage RNA Polymerase. K.D. MacFerrin, L. Chen, M.P. Terranova, S.L. Schreiber, and G.L. Verdine, Overproduction of Proteins Using Expression-Cassette Polymerase Chain Reaction. C. Coleclough, Cell-Free Expression of Large Collections of cDNA Clones Using Positive-Selection lambda Phage Vectors. Mutagenesis and Gene Disruption: L.-J. Zhao, Q.X. Zhang, and R. Padmanabhan, Polymerase Chain Reaction-Based Point Mutagenesis Protocol. G.P. Smith and J.K. Scott, Libraries of Peptides and Proteins Displayed on Filamentous Phage. R.M. Horton, S.N. Ho, J.K. Pullen, H.D. Hunt, Z. Cai, and L.R. Pease, Gene Splicing by Overlap Extension. T.C. Huffaker, Expression and Screening in Yeast of Genes Mutagenized in Vitro . Screening Libraries, Identifying and Mapping Genes: J. Chao, K.X. Chai, and L. Chao, Screening of Complementary DNA Library Using Radiolabeled Antigen. G.V. Paddock, Rapid Colony Hybridization on Whatman 541 Paper Using Oligonucleotide Probes. A. Poustka, Large Insert Linking-Clone Libraries: Construction and Use. A. Poustka, Construction and Use of Chromosome-Jumping Libraries. I. Bronstein, J.C. Voyta, O.J. Murphy, R. Tizard, C.W. Ehrenfels, and R.L. Cate, Detection of DNA in Southern Blots with Chemiluminescence. V. Shyamala and G.F.-L. Ames, Genome Walking by Single Specific Primer-Polymerase Chain Reaction. Methods for Transforming Animal and Plant Cells: H. Potter, Application of Electroporation in Recombinant DNA Technology. J.C. Sanford, F.D. Smith, and J.A. Russell, Optimizing the Biolistic Process for Different Biological Applications. A.D. Miller, D.G. Miller, J.V. Garcia, and C.M. Lynch, Use of