Regulators and Effectors of Small GTPases, Part E: GTPases Involved in Vesicular Traffic

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ISBN: 9780121822309 | 0121822303
Cover: Hardcover
Copyright: 1/25/2001

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Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport. This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.

Contributors to Volume 329

Preface

xvii

Volumes in Series

xix

Section I. Rab GTPases

Structural Basis for Rab Function: An Overview

(3)

Bryan D. Moyer

William E. Balch

Expression of Wild-Type and Mutant Green Fluorescent Protein--Rab1 for Fluorescence Microscopy Analysis

(8)

Bryan D. Moyer

Jeanne Matteson

William E. Balch

Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase

(17)

Kirill Alexandrov

Axel J. Scheidig

Roger S. Goody

Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases

(8)

Jean H. Overmeyer

William A. Maltese

Antibody and Oligonucleotide Probes to Distinguish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor Isoforms

(11)

Assia Shisheva

Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family

(9)

Elke Will

Stefan Albert

Dieter Gallwitz

Purification and Properties of Rab3 GDP/GTP Exchange Protein

(8)

Hiroyuki Nakanishi

Yoshimi Takai

Purification and Properties of Rab3 GTPase-Activating Protein

(8)

Fumiko Nagano

Takuya Sasaki

Yoshimi Takai

Rabphilin-3: A Target Molecule for Rab3 Small G Proteins

(8)

Hiromichi Shirataki

Takuya Sasaki

Yoshimi Takai

Doc2α as Modulator of Ca2+-Dependent Exocytosis

(8)

Satoshi Orita

Takuya Sasaki

Yoshimi Takai

Purification and Properties of Sec4p GTPase-Activating Protein

(9)

Li-Lin Du

Peter Novick

Purification and Characterization of Yeast Exocyst Complex

100

(11)

Daniel R. TerBush

Wei Guo

Steven Dunkelbarger

Peter Novick

Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling

111

(9)

Peter Van Der Sluijs

Karin Mohrmann

Magda Deneka

Mandy Jongeneelen

Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays

120

(12)

Savvas Christoforidis

Marino Zerial

Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-5

132

(13)

Roger Lippe

Hisanori Horiuchi

Anja Runge

Marino Zerial

Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis

145

(12)

Manuel A. Barbieri

A. Gumusboga

Richard L. Roberts

Philip D. Stahl

Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6A

157

(8)

Arnaud Echard

Ahmed El Marjou

Bruno Goud

Expression and Functional Analyses of Rab8 and Rab11a in Exocytic Transport from trans-Golgi Network

165

(10)

Wei Chen

Angela Wandinger-Ness

Expression and Properties of Rab7 in Endosome Function

175

(13)

Yan Feng

Barry Press

Wei Chen

Jay Zimmerman

Angela Wandinger-Ness

Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport

188

(9)

John Peranen

Johanna Furuhjelm

Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase δ Subunit

197

(13)

Anne-Marie Marzesco

Thierry Galli

Daniel Louvard

Ahmed Zahraoui

Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate Filters

210

(15)

Peter J. Peters

Walter Hunziker

Expression and Properties of Rab25 in Polarized Madin--Darby Canine Kidney Cells

225

(9)

James R. Goldenring

Lorraine M. Aron

Lynne A. Lapierre

Jennifer Navarre

James E. Casanova

Purification of TRAPP from Saccharomyces cerevisiae and Identification of Its Mammalian Counterpart

234

(11)

Michael Sacher

Susan Ferro-Novick

Section II. ADP-Ribosylation Factor (ARF) GTPases

Structural and Functional Organization of ADP-Ribosylation Factor (ARF) Proteins

245

(2)

Sophie Beraud-Dufour

William E. Balch

Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathway

247

(9)

Julie G. Donaldson

Harish Radhakrishna

Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation Factor (AFR)-Mediated Actin Cytoskeletal Rearrangements

256

(8)

Lorraine C. Santy

Scott R. Frank

James E. Casanova

Expression, Purification, and Measurements of Activity of ARNO1, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factor 1 (ARF1)

264

(8)

Sophie Beraud-Dufour

Sylviane Robineau

Expression, Purification, and Biochemical Properties of EFA6, a Sec7 Domain-Containing Guanine Exchange Factor for ADP-Ribosylation Factor 6 (ARF6)

272

(7)

Philippe Chavrier

Michel Franco

Isolation and Properties of GRP1, an ADP-Ribosylation Factor (ARF)-Guanine Nucleotide Exchange Protein Regulated by Phosphatidylinositol 3,4,5-Trisphosphate

279

(11)

Jes K. Klarlund

Michael P. Czech

Functional Analysis of ADP-Ribosylation Factor (ARF) Guanine Nucleotide Exchange Factors Gea1p and Gea2p in Yeast

290

(10)

Anne Peyroche

Catherine L. Jackson

Isolation, Cloning, and Characterization of Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein for ADP-Ribosylation Factor

300

(7)

Gustavo Pacheco-Rodriguez

Joel Moss

Martha Vaughan

Expression, Purification, and Properties of ADP-Ribosylation Factor (ARF) GAP1

307

(10)

Irit Huber

Miriam Rotman

Elah Pick

Vardit Makler

Lilah Rothem

Edna Cukierman

Dan Cassel

Expression, Analysis, and Properties of Yeast ADP-Ribosylation Factor (ARF) GTPase Activating Proteins (GAPs) Gcs1 and Glo3

317

(7)

Pak Phi Poon

Dan Cassel

Irit Huber

Richard A. Singer

Gerald C. Johnston

Purification and Properties of ARD1, an ADP-Ribosylation Factor (ARF)-Related Protein with GTPase-Activating Domain

324

(11)

Nicolas Vitale

Joel Moss

Martha Vaugflan

Purification and Characterization of GIT Family of ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins

335

(8)

Richard T. Premont

Nicolas Vitale

Assay and Purification of Phosphoinositide-Dependent ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins

343

(12)

Paul A. Randazzo

Koichi Miura

Trevor R. Jackson

Biological Properties and Measurement of Phospholipase D Activation by ADP-Ribosylation Factor (ARF)

355

(17)

Shamshad Cockcroft

Kun Bi

Nicholas T. Ktistakis

Michael G. Roth

Use of Aminoglycoside Antibiotics and Related Compounds to Study ADP-Ribosylation Factor (ARF)/Coatomer Function in Golgi Traffic

372

(7)

Rockford K. Draper

Robert Tod Hudson

Tonghuan Hu

Adaptor Protein 1-Dependent Clathrin Coat Assembly on Synthetic Liposomes and Golgi Membranes

379

(9)

Yunxiang Zhu

Matthew T. Drake

Stuart Kornfeld

Receptor-Dependent Formation of COPI-Coated Vesicles from Chemically Defined Donor Liposomes

388

(17)

Walter Nickel

Felix T. Wieland

ADP-Ribosylation Factor (ARF) as Regulator of Spectrin Assembly at Golgi Complex

405

(12)

Maria Antonietta De Matteis

Jon S. Morrow

Purification, Properties, and Analysis of yARL3

417

(7)

Fang-Jen S. Lee

Chun-Fang Huang

Preparation and Assay of Recombinant ADP-Ribosylation Factor-Like Protein-1 (ARL1)

424

(7)

Gustavo Pacheco-Rodriguez

Joel Moss

Martha Vaughan

Section III. Sar GTPases

Purification and Properties of Rat Liver Sec23-Sec24 Complex

431

(7)

Jacques T. Weissman

Meir Aridor

William E. Balch

Purification of Functional Sec13p--Sec31p Complex, A Subunit of COPII Coat

438

(9)

William J. Belden

Charles Barlowe

Section IV. Dynamin GTPases

Expression, Purification, and Functional Assays for Self-Association of Dynamin-1

447

(10)

Hanna Damke

Amy B. Muhlberg

Sanja Sever

Steven Sholly

Dale E. Warnock

Sandra L. Schmid

Analysis of Phosphoinositide Binding by Pleckstrin Homology Domain from Dynamin

457

(11)

Anthony Lee

Mark A. Lemmon

Mapping Dynamin Interdomain Interactions with Yeast Two-Hybrid and Glutathione S-Transferase Pulldown Experiments

468

(10)

Elena Smirnova

Dixie-Lee Shurland

Alexander M. Vander Bliek

Interaction of Dynamin and Amphiphysin with Liposomes

478

(8)

Kohji Takei

Vladimir I. Slepnev

Pietro De Camilli

Activation of Dynamin GTPase Activity by Phosphoinositides and SH3 Domain-Containing Proteins

486

(13)

Barbara Barylko

Derk D. Binns

Joseph P. Albanesi

Section V. Septin GTPases

Expression and Analysis of Properties of Septin CDCrel-1 in Exocytosis

499

(12)

Crestina L. Bettes

Xiao-Rong Peng

William S. Trimble

Author Index

511

(26)

Subject Index

537

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Regulators and Effectors of Small GTPases, Part E: GTPases Involved in Vesicular Traffic

Summary

Table of Contents

Supplemental Materials

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Regulators and Effectors of Small GTPases, Part E: GTPases Involved in Vesicular Traffic

Summary

Table of Contents

Supplemental Materials

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