RNA Interference Technology: From Basic

RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this 2005 overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.

Basic RNAi, siRNA and Gene Silencing Mechanisms
RNAi beginnings: overview of the pathway in C. elegans
Dicer in RNAi: its roles in vivo and utility in vitro
Genes required for RNAi
MicroRNAs: a small contribution from worms
miRNAs in the brain and the application of RNAi to neurons;
Design and Synthesis of siRNAs
Design and synthesis of small interfering RNA (siRNA)
Automated design and high throughput synthesis of siRNA
Rational design of siRNAs with the Sfold software
Enzymatic production of siRNAS;
Vector Development and In Vivo, In Vitro and In Ovo Delivery Methods
Six methods of inducing RNAi in mammalian cells
Viral delivery of siRNA
siRNA delivery by lentiviral vectors: design and applications
Liposomal delivery of siRNAs in mice
Chemical modifications to achieve increased stability and sensitive detection of siRNA
RNAi in postimplantation mouse embryos
In ovo RNAi opens new possibilities for functional genomics in vertebrates;
Gene Silencing in Model Organisms
Practical applications of RNAi in C. elegans
Inducible RNAi as a forward genetic tool in Trypanosoma brucei
RNAi gene silencing in yeast
RNA silencing in filamentous fungi: Mucor circinelloides as a model organism
RNAi and gene silencing phenomenon mediated by viral suppressors in plants;
Drug Target Validation
Delivering siRNA in vivo for functional genomics and novel therapeutics
The role of RNAi in drug target validation: application to Hepatitis C
RNAi in the drug discovery process
RNAi: use in the drug discovery process;
Therapeutic and Drug Development
RNAi-mediated silencing of viral gene expression and replication
RNAi in drug development: practical considerations
RNAi for the treatment of liver diseases
RNAi applications in animal systems;
Genome Wide RNAi and High Through-Put Analysis
High through-put RNAi-by-soaking in Caenorhabditis elegans
Tools for integrative genomics: genome-Wide RNAi and expression profiling in Drosophila
Microarray analysis and RNA silencing to determine genes functionally important in Mesothelioma
High through-put RNAi
Generation of highly specific vector-based shRNAi libraries directed against the entire human genome
Table of Contents provided by Publisher. All Rights Reserved.

What is included with this book?

The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

The Used, Rental and eBook copies of this book are not guaranteed to include any supplemental materials. Typically, only the book itself is included. This is true even if the title states it includes any access cards, study guides, lab manuals, CDs, etc.

FREE SHIPPING

Amazon no longer offers textbook rentals. We do!

RNA Interference Technology: From Basic Science to Drug Development

Summary

Table of Contents

Supplemental Materials