Species Diagnostics Protocols

In Species Diagnostics Protocols leading practitioners describe in full detail numerous methods for identifying many organisms by means of nucleic acid analysis. The applications range from medical diagnosis and plant pathology to medical entomology, mycology, and molecular ecology. The organisms identified include plant, insect, and vertebrate DNA; viruses (phytopathogenic, HIV, human papilloma, dengue); bacteria; fungi; protozoans; parasites; and mosquitoes. The detailed techniques can easily be modified for use with many other and novel species. Species Diagnostics Protocols is the only book available dealing solely with the identification of species using molecular techniques. Its detailed, step-by-step instructions and extensive notes on pitfalls ensure success even for those with little prior experience.

Isolation and Purification of Plant Nucleic Acids: Genomic and Chloroplast DNA. Isolation and Purification of Insect DNA. Isolation and Purification of Vertebrate DNAs. Statistical Analysis of Arbitrarily Primed PCR Patterns in Molecular Taxonomic Studies. Molecular Identification of Phytopathogenic Viruses. Improved PCR Methods for Identification of Phytopathogenic Viruses. PCR Detection of HIV. The Use of Consensus PCR and Direct Sequence Analysis for the Identification of HPV. The Identification of Dengue Virus Using PCR. The Design and Application of Ribosomal RNA-Targeted Fluorescent Oligonucleotide Probes for the Identification of Endosymbionts in Protozoa. Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes. The Use of RAPD for Generating Specific DNA Probes for Microorganisms. ITS-RFLP Matching for Identification of Fungi. Specific PCR Primers for the Identification of Endomycorrhizal Fungi. Single-Strand Conformational Polymorphism (SSCP) for the Identification of Endomycorrhizal Fungi. The Use of RAPD for Isolate Identification of Arbuscular Mycorrhizal Fungi. Establishing Relationships Between Closely Related Species Using Total Genomic DNA as a Probe. Detection and Characterization of Leishmania Parasites by DNA- Based Methods: Southern Blotting and PCR. The Molecular Identification of Trypanosomes. Detection and Identification of the Four Malaria Parasite Species Infecting Humans by PCR Amplification. The Use of Degenerate Primers in Conjunction with Strain and Species Oligonucleotides to Classify Onchocerca volvulus. The Use of Synthetic DNA Probes for the Field Identification of Members of the Anopheles gambiae Complex. PCR of the Ribosomal DNA Intergenic Spacer Regions as a Method for Identifying Mosquitoes in the Anopheles gambiae Complex. Restriction Fragment Length Polymorphism (RFLP) Analyses: Distinguishing African and European Honey Bees. Differential Screening for the Isolation of Species-Specific Sequences in the Anopheles farauti Complex. RAPD- PCR with Parasitic Hymenoptera. The Use of Selective Enrichment for the Isolation of Species- Specific DNA Probes for Insects. Index.