Stem Cell Culture

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ISBN: 9780123738769 | 0123738768
Cover: Hardcover
Copyright: 5/15/2008

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Interest in the therapeutic value of embryonic, fetal and adult stem cell types is rapidly expanding throughout the scientific community. The field of stem cell culture is made more challenging, and thus more exciting, by the special properties of these fascinating cells which can both self-renew and differentiate into many types of cells in the adult. The first half of this century should see an explosion of therapeutic applications of stem cells which will grow from the cells and techniques described in this book.

Contributors	p. xi
Preface	p. xv
Biography	p. xix
Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions
Introduction	p. 1
Methods	p. 2
Discussion	p. 12
Materials	p. 12
References	p. 13
Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder-Free Conditions
Introduction	p. 16
Methods and Materials	p. 17
Results	p. 21
Discussion and Summary	p. 25
References	p. 28
Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation
Introduction	p. 30
Rationale	p. 32
Methods	p. 32
Summary	p. 54
References	p. 55
Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal
Introduction	p. 60
Rationale	p. 63
Methods	p. 67
Materials	p. 78
Discussion	p. 80
References	p. 81
Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability
Introduction	p. 86
Amniotic Fluid Cell Composition and Stem Cells	p. 87
In Vitro Potential of Amniotic Fluid Stem Cells	p. 91
Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells	p. 94
Discussion	p. 97
References	p. 98
Method to Isolate Mesenchymal-Like Cells from Wharton's Jelly of Umbilical Cord
Introduction	p. 102
Rationale	p. 103
Methods	p. 103
Materials	p. 110
UCMSCs and Culture Characteristics	p. 115
Discussion	p. 116
References	p. 118
Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs)
Introduction	p. 122
Methods	p. 123
Concluding Remarks	p. 133
References	p. 134
Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance
Introduction	p. 139
Epithelial-Mesenchymal Relationship	p. 139
Stem Cells and Lineage Biology	p. 140
Liver Processing	p. 150
Fractionation of Liver Cell Subpopulations	p. 162
Feeder Cells	p. 170
Extracellular Matrix	p. 174
Culture Media	p. 176
Monolayer Cultures of Cells	p. 180
Three-Dimensional Systems	p. 185
Nomenclature, Glossary, and Abbreviations	p. 195
Details on the Preparation of Buffers/Reagents	p. 207
Commercial Sources of Reagents	p. 211
Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells	p. 212
References	p. 213
Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo
Introduction	p. 228
Rationale	p. 229
Methods	p. 229
Materials	p. 237
Discussion	p. 237
Summary	p. 238
References	p. 239
Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells
Introduction	p. 242
Rationale	p. 243
Methods	p. 245
Materials	p. 250
Discussion	p. 253
Summary	p. 254
References	p. 254
Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue
Introduction	p. 258
Isolation of hMSCs from Trabecular Bone	p. 259
Isolation of hMSCs from Adipose Tissue	p. 263
Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs	p. 265
Surface Marker Characterization of hMSCs	p. 267
hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways	p. 270
Summary	p. 276
References	p. 276
Culture of Mesenchymal Stem/Progenitor Cells in Adhesion-Independent Conditions
Introduction	p. 280
Suspension Culture of Bone Marrow-Derived MPCs	p. 280
Analysis of Suspension Culture	p. 283
Mechanism of MPC Expansion in Suspension	p. 288
Discussion	p. 291
References	p. 292
Purification and Long-Term Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes
Introduction	p. 297
Materials and Methods: Cell Analysis and Purification by Flow Cytometry	p. 298
Materials and Methods: Long-Term Culture of Human Blood Vessel-Associated Progenitor Cells	p. 301
Materials and Methods: Proliferation Kinetics of Human Myoendothelial Cells and Pericytes	p. 303
Materials and Methods: Genotypic and Phenotypic Analyses of Long-Term Cultured Cells	p. 304
Conclusion	p. 306
References	p. 308
Isolation and Culture of Colon Cancer Stem Cells
Introduction	p. 312
Identification of CSCs in Colon Cancer Tissue	p. 314
Isolation and Propagation of Colon CSCs	p. 315
Discussion	p. 322
References	p. 323
Isolation and Establishment of Human Tumor Stem Cells
Introduction	p. 326
Rationale	p. 327
Methods	p. 327
Results	p. 336
Discussion	p. 338
Materials	p. 340
References	p. 341
Stem Cells from Cartilaginous and Bony Fish
Introduction to Models and Uses	p. 344
Embryonal Stem Cells	p. 348
Tissue-Specific Stem Cells	p. 353
Outlook and Future Contributions	p. 361
References	p. 362
Index	p. 369
Volumes in Series	p. 379
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