Techniques in Glycobiology

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ISBN: 9780824798222 | 0824798228
Cover: Hardcover
Copyright: 6/25/1997

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Covers state-of-the-art methodologies for plant & animal glycoconjugate analysis--detailing mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids & new physical methods, O-glycosylation characterization, chromophore & flourophore labeling of oligosaccharides, separations, exoglycosidases & mapping, & plant glycobiology.

Preface

iii

(10)

Contributors

xiii

Part I Mass Spectrometry and NMR

(110)

1. New Frontiers in Nuclear Magnetic Resonance Spectroscopy: Use of the Nano NMR Probe for the Analysis of Microgram Quantities of Complex Carbohydrates

(16)

Adriana E. Manzi

Paul A. Keifer

2. Analysis of Anionic Glycoconjugates by Delayed Extraction Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry

(16)

Bradford W. Gibson

Jeffrey J. Engstrom

Constance M. John

Arnold M. Falick

Wade M. Hines

Stephen A. Martin

3. Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry of Oligosaccharides Separated by High pH Anion-Exchange Chromatography

(20)

Damon I. Papac

Andrew J. S. Jones

Louisette J. Basa

4. The Fragmentation Behavior of Glycopeptides Using the Post-Source Decay Technique and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

(14)

Uwe Rapp

Anja Resemann

Franz J. Mayer-Posner

Wolfram Schafer

Konrad Feichtinger

5. Application of Electrospray Ionization Mass Spectrometry to Unusual Phosphorylated Oligosaccharides of Trypanosoma cruzi

(10)

Paul A. Haynes

George A. M. Cross

Michael A. J. Ferguson

6. Analysis of Carbohydrate-Protein Interactions by Mass Spectrometry

(8)

Yoko Ohashi

Sadamu Kurono

Yoshitaka Nagai

7. Mycobacterial Lipoarabinomannans: Structural and Tagging Studies

(26)

Carole Delmas

Anne Venisse

Alain Vercellone

Martine Gilleron

Renaud Albigot

Therese Brando

Michel Riviere

Germain Puzo

Part II Glycolipids and New Physical Methods

111

(136)

8. Microscale Analysis of Glycosphingolipids by Thin-Layer Chromatography Blotting

111

(16)

Takao Taki

9. High-Performance Liquid Chromatography of Gangliosides

127

(10)

Sandro Sonnino

Manuela Valsecchi

Paola Palestini

Marco Nicolini

Vanna Chigorno

10. A Sensitive Cell Suspension Enzyme-Linked Immunosorbent Assay Measuring the Density of Cell Surface Ganglioside and Carbohydrate Antigens

137

(30)

Mepur H. Ravindranath

Amir A. Amiri

Philip M. Bauer

11. Scattering Techniques for the Study of Aggregative Properties of Gangliosides

167

(20)

Laura Cantu

Mario Corti

Elena Del Favero

12. Imaging Carbohydrate Polymers with Noncontact Mode Atomic Force Microscopy

187

(22)

Theresa M. McIntire

David A. Brant

13. Novel Approach for the Analysis of Interaction Between Sugar Chains and Carbohydrate-Recognizing Molecules Using a Biosensor Based on Surface Plasmon Resonance

209

(20)

Yasuro Shinohara

Hiroyuki Sota

Yukio Hasegawa

14. Glycoprotein Detection Using the Light-Addressable Potentiometric Sensor

229

(18)

Kilian Dill

Part III Protein O-Glycosylation

247

(102)

15. Effects of O-Glycosylation on Protein Structure: The Role and Characterization of Heavily O-Glycosylated Mucin Domains

247

(24)

Thomas A. Gerken

Cheryl L. Owens

Murali Pasumarthy

16. Predicting Mucin-Type O-Glycosylation Sites

271

(18)

Ake P. Elhammer

Roger A. Poorman

Ferenc J. Kezdy

17. Experimental Approaches to Studying O-Glycosylation In Vivo

289

(10)

Keith Nehrke

Fred K. Hagen

Lawrence A. Tabak

18. Identifying Sites of Glycosylation in Proteins

299

(22)

Anthony Pisano

Daniel R. Jardine

Nicolle H. Packer

John W. Redmond

Keith L. Williams

Andrew A. Gooley

Vince Farnsworth

Wulf Carson

Paul K. Cartier III

19. High Energy Collision-Induced Dissociation of O-Linked Glycopeptides

321

(14)

Katalin F. Medzihradszky

20. Analysis of Permethylated Glycoprotein Oligosaccharide Fractions by Gas Chromatography and Gas Chromatography-Mass Spectrometry

335

(14)

Kristina A. Thomsson

Niclas G. Karlsson

Hasse Karlsson

Gunnar C. Hansson

Part IV Labeling Oligosaccharides with Chromophores and Fluorophores

349

(52)

21. Highly Sensitive Pre-Column Derivatization Procedures for Quantitative Determination of Monosaccharides, Sialic Acids, and Amino Sugar Alcohols of Glycoproteins by Reversed Phase High-Performance Liquid Chromatography

349

(10)

Kalyan Rao Anumula

22. Glycan Labeling with the Fluorophores 2-Aminobenzamide and Anthranilic Acid

359

(18)

Mark R. Hardy

23. Capillary Gel Electrophoresis of 8-Aminopyrene-3,6,8-trisulfonate-Labeled Oligosaccharides

377

(14)

Andras Guttman

24. Evaluation of Glycosylpyrazole Derivatives in Carbohydrate Analysis

391

(10)

Warren C. Kett

Michael Batley

John W. Redmond

Part V Strategies for Oligosaccharide Separations and Sequencing

401

(132)

25. Discovery and Uses of Novel Glycosidases

401

(8)

Sharon T. Wong-Madden

David Landry

Ellen P. Guthrie

26. Resolution of Oligosaccharides in Capillary Electrophoresis

409

(22)

Morgan Stefansson

Milos Novotny

27. A Comparison of Oligosaccharide Profiling Methods

431

(12)

Elizabeth Higgins

Richard Bernasconi

28. High-Performance Liquid Chromatographic Mapping of Oligosaccharides Using High pH Anion-Exchange Chromatography: Improvements from Sample Preparation, Reduction, and Fluorometric Detection

443

(14)

Peter H. Lipniunas

Lorri Reinders

R. Reid Townsend

James Bruce

Collette Bigge

Raj Parekh

29. Monosaccharide Analysis of Recombinant Glycoproteins by High pH Anion-Exchange Chromatography/High-Performance Liquid Chromatography Using a Refractive Index Detector

457

(18)

M. Janardhan Rao

Yong K. Cho

Roderic P. Kwok

30. 2-Aminobenzamide Labeling, Matrix-Assisted Laser Desorption A Sensitive Method for Monitoring Lot-to-Lot Consistency of Recombinant Glycoproteins

475

(16)

Kristina Kopp

Michael Schluter

Rolf G. Werner

31. Characterization of Subnanomolar Amounts of N-Glycans by 2-Aminobenzamide Labeling, Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, and Computer-Assisted Sequence Analysis

491

(18)

Rolf Nuck

Martin Gohlke

32. Isolation and Characterization of N-Linked Oligosaccharides from Yeast Glycoproteins

509

(24)

Robert B. Trimble

Part VI Plant Glycobiology

533

(96)

33. High Resolution Electrophoretic Analysis of Carbohydrates Using a DNA Sequencer

533

(20)

Michael G. O'Shea

Matthew Morrell

34. Recent Developments in Starch Structure Analysis Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

553

(14)

Kit-Sum Wong

Jay-lin Jane

35. Red Seaweed Galactans: Methodology for the Structural Determination of Corallinan, a Different Agaroid

567

(28)

Carlos A. Stortz

Marcelo R. Cases

Alberto Cerezo

36. Some New Methods to Study Plant Polyuronic Acids and Their Esters

595

(18)

Nicholas C. Carpita

Maureen C. McCann

37. Analysis of Red Algal Extracellular Matrix Polysaccharides: Cellulose and Carrageenan

613

(16)

Michael R. Gretz

Jean-Claude Mollet

Ruth Falshaw

Index

629

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Techniques in Glycobiology

Summary

Table of Contents

Supplemental Materials