Techniques in Protein Chemistry VIII

Praise for the Series: "The mainly sharp scientific focus of this set of snapshots is a credit to both the contricutors and the editorial team." --Biotechnology and Applied Biochemistry Techniques in Protein Chemistry VIII is the latest volume in this successful series. As a valuable bench-top reference tool for protein chemists, the ten section sof the book are divided by subject area to show the reader which techniques are currently applied to particular problems in protein science. This approach reflects current trends in which specific instruments and methodologies are used in several different areas. * * The book features the latest advances in protein chemistry methodologies in the following areas: * Protein sequencing and amino acid analysis * Mass spectral analysis of peptides and proteins * Posttranslational processing * High-sensitivity protein and peptide separations * Protein folding and NMR * Functional domain analysis * Protein design and engineering * Three-dimensional protein structure

Foreword
Preface
Acknowledgments
Primary Structural Analysis
Protein Sequencing Using Microreactors and Capillary Electrophoresis with Thermo-optical Absorbance Detection
Enhancement of Concentration Limits of Detection in Capillary Electrophoresis
Examples of On-Line Sample Preconcentration, Cleanup, and Microreactor Technology in Protein Characterization
Sequencing MHC Class I Peptides Using Membrane Preconcentration-Capillary Electrophoresis Tandem Mass Spectrometry (mPC-CE-MS/MS)
Nano-electrospray Mass Spectrometry and Edman Sequencing of Peptides and Proteins Collected from Capillary Electrophoresis
Characterization of a Recombinant Hepatitis E Protein Vaccine Candidate by Mass Spectrometry and Sequencing Techniques
Comparison of the High Sensitivity and Standard Versions of Applied Biosystems Procise TM 494 N-Terminal Protein Sequencers Using Various Sequencing Supports
Evaluation of ABRF-96SEQ: A Sequence Assignment Exercise
Internal Protein Sequencing of SDS-PAGE-Separated Proteins: Optimization of an In Gel Digest Protocol
A Strategy to Obtain Internal Sequence Information from Blotted Proteins after Initial N-terminal Sequencing
Internal Protein Sequencing of SDS PAGE-Separated Proteins: A Collaborative ABRF Study
Physical and Chemical Analysis
Chromatographic Determination of Extinction Coefficients of Non-Glycosylated Proteins Using Refractive Index (RI) and UV Absorbance (UV) Detectors
Applications for Studying Protein Interactions by Size Exclusion Chromatography with Light-Scattering, UV, and RI Detectors
Single Alkaline Phosphatase Molecule Assay by Capillary Electrophoresis Laser-Induced Fluorescence Detection
A New Centrifugal Device Used in Sample Clean-up and Concentration of Peptides
Sample Preparation Using Synthetic Membrances for the Study of Biopolymers by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry
Use of LC/MS Peptide Mapping for Characteriation of Isoforms in 15 N-Labeled Recombinant Human Leptin
Hyphenated HPLC Methodology for the Resolution and Elucidation of Peptides from Proteolytic Digests
Detecting and Identifying Active Compounds from a Combinatorial Library Using Iasys and Electrospray Mass Spectrometry
Amino Acid Analysis of Unusual and Complex Samples Based on 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization
Development of a Method for Analysis of Free Amino Acids from Physiological Samples Using a 420A ABI/PE Amino Acid Analyzer
Quantitation and Identification of Proteins by Amino Acid Analysis
ABRF-96AAA Collaborative Trial Chemical Modification: Nonaqueous Chemical Modification of Lyophilized Proteins Reaction of HIV-1 NC p7 Zinc Fingers with Electrophilic Reagents
The Identification and Isolation of Reactive Thiols in Ricin A-Chain and Blocked Ricin Using 2-(4'-Maleimidylanilino)naphthalene-6-sulfonic Acid
Inactivation of the Human Cytomegalovirus Protease by Diisopropylfluorophosphate
Studies on the Status of Arginine Residues in Phospholipase A 2 from Naja naja atra (Taiwan cobra) Snake Venom
Selective Reduction of the Intermolecular Disulfide Bridge in Human Glial Cell Lined-Derived Neurotrophic Factor Using Tris-(2-Carboxyethyl)Phosphine
Effects of Surface Hydrophobicity on the Structural Properties of Insulin
The Effects of in Vitro Methionine Oxidation on the Bioactivity and Structure of Human Keratinocyte Growth Factor
Posttranslational and Other Modifications: Effects of Enzyme Glycosylation on the Chemical Step of Catalysis, as Probed by Hydrogen Tunneling and Enthalpy of Activation
Profile Analysis of Oligosaccharides from Glycoproteins by PMP Labeling
Comparison of Chemical and Enzymatic Release Methods Using RP-HPLC and Mass Spectrometry
Positive Identification of Glycosylation Sites in Proteins and Peptides Using a Modified Beckman LF 3600
Table of Contents provided by Publisher. All Rights Reserved.

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Techniques in Protein Chemistry VIII

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