Tumor Necrosis Factor

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ISBN: 9781588292230 | 1588292231
Cover: Hardcover
Copyright: 3/1/2004

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A comprehensive collection of readily reproducible techniques for TNF production, characterization, mutagenesis, and detection in biological specimens. Also included are several in vitro assays and animal models for studying the role of TNF in various TNF-related diseases and in cancer. The collection offers molecular and cellular biologists, pharmacologists, and toxicologists a diverse set of versatile, cutting-edge tools for illuminating the pathophysiological roles of TNF in disease and for identifying new drugs.

Preface

Contributors

1 Tumor Necrosis Factor as a Pharmacological Target

Pietro Ghezzi and Anthony Cerami

(8)

2 Production and Characterization of Recombinant Human and Murine TNF

Flavio Curnis and Angelo Corti

(14)

3 Purification of TNF Binding Proteins

Hartmut Engelmann, Dani Aderka, and David Wallach

(10)

4 Production and Characterization of Receptor-Specific TNF Muteins

Paul Ameloot and Peter Brouckaert

(14)

5 TNFa Promoter Polymorphysms

Silvia Fargion, Luca Valenti, Paola Dongiovanni, and Anna Ludovica Fracanzani

(12)

6 Detection of TNF and TNF Receptor mRNA in Cells and Tissues

Roberto Furlan, Giuliana Salani, Alessandra Bergami, and Gianvito Martino

(14)

7 Immunohistochemical Localization of TNFa and Its Receptors in the Rodent Central Nervous System

Paolo Bigini and Tiziana Mennini

(8)

8 TNF Signaling: Key Protocols

Marta Muzio and Simona Saccani

(20)

9 More Than One Way to Die: Methods to Determine TNF-Induced Apoptosis and Necrosis

Tom Vanden Berghe, Geertrui Denecker, Greet Brouckaert, Dmitri Vadimovisch Krysko, Katharina D'Herde, and Peter Vandenabeele

101

(26)

10 Assessment of TNFa-Induced Endothelial Damage Through the Loss of Its Barrier Function

Elisabetta Ferrero

127

(10)

11 Genetic Engineering in the Mouse: Tuning TNF/TNFR Expression

Eleni Douni, Maria Alexiou, and George Kollias

137

(34)

12 TNFa in Experimental Diseases of the CNS

Roberto Furlan, Pia Villa, Giorgio Senaldi, and Gianvito Martino

171

(20)

13 In Vivo Vascular Leakage Assay

Maria Elena Ferrero

191

(8)

14 Animal Models of Endotoxic Shock

Pia Villa and Pietro Ghezzi

199

(8)

15 Collagen-Induced Arthritis as a Model for Rheumatoid Arthritis

Richard O. Williams

207

(10)

16 Myocardial Infarction: Animal Models

Stefano Chimenti, Eleonora Carlo, Serge Masson, Antonio Bai, and Roberto Latini

217

(10)

17 Tumor Vascular Therapy With TNF: Critical Review on Animal Models

Timo L. M. ten Hagen and Alexander M. M. Eggermont

227

(20)

18 Strategies for Improving the Anti-Neoplastic Activity of TNF by Tumor Targeting

Angelo Corti

247

(18)

Index

265

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Tumor Necrosis Factor

Summary

Table of Contents

Supplemental Materials

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Tumor Necrosis Factor

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Table of Contents

Supplemental Materials

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