Stevioside Technology, Applications and Health

Stevioside is one of the naturally occurring sweeteners, belonging to the diterpene glycoside family, which can be widely applied in food, drinks, medicine and consumer chemicals. It is a good dietary supplement, being non-calorific, thermally stable, non-toxic, with a sugar-like taste profile, and suitable for diabetics, phenylketonuria patients and the obese. It is also non-fermentable, and exhibits anti-carcinogenic, antioxidant and anti-hyperglycemic properties. Stevioside tastes about 300 times sweeter than 0.4% sucrose solution. Thus, it offers a reasonably rare combination of health benefits and taste benefits, making the extraction of stevioside is an area of active research for the food industry as well as academic food scientists.With the rapid increase in the popularity of stevioside as a sugar substitute, particularly with regard to its associated health benefits, there is a need for more efficient and feasible extraction processes for stevioside in the near future. 

This book offers an in-depth look at perhaps the major “sugar alternative of the future”, up-to-date and in line with the latest global legislation regarding its suitability for consumption and its scope for application. 

Sirshendu De is a professor in the Department of Chemical Engineering, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India.

Sourav Mondal is a researcher in the Department of Chemical Engineering, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India.

Suvrajit Banerjee was Master of Technology in 2012 at the Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India.

Preface ix

Acknowledgements xi

List of figures xiii

List of tables xvii

1 Introduction to stevioside 1

1.1 History of Stevia 3

1.2 Composition of Stevia 6

1.3 Source of stevioside 10

1.4 Physicochemical and biological properties of steviol glycosides 13

1.5 Analysis of stevioside (steviol glycosides) and Stevia extract 18

2 Health benefits and pharmacological effects of steviol glycosides 27

2.1 Effect of stevioside in absorption, distribution, metabolism and excretion 27

2.2 Antihyperglycaemic effect 29

2.3 Antihypertensive effect 31

2.4 Anti-inflammatory effect 32

2.5 Anticarcinogenic antitumour effects 33

2.6 Antioxidant activity 34

2.7 Antimicrobial and antidiarrhoeal effects 34

2.8 Effect on renal function 35

3 Applications of stevioside 45

4 Conventional extraction processes of stevioside 51

4.1 Ion exchange 51

4.2 Solvent extraction 53

4.3 Extraction by chelating agents 54

4.4 Adsorption and chromatographic separation 56

4.5 Ultrasonic extraction 59

4.6 Microwave-assisted extraction 59

4.7 Supercritical fluid extraction 60

5 Brief introduction to pressure-driven membrane-based processes 65

5.1 Advantages of the membrane-based process 66

5.2 Classification of the processes 66

5.3 Characterisation of membranes 67

5.4 Membrane modules 70

5.5 Limitations 73

5.6 Quantification of concentration polarisation 74

5.7 Applications of membrane-based processes 79

6 State of the art of stevioside processing using membrane-based filtration 91

6.1 Clarification and purification 92

6.2 Concentration by nanofiltration 95

6.3 Limitations 97

7 Detailed membrane-based technologies for extraction of stevioside 99

7.1 Outline of processing 99

7.2 Optimisation of water extraction process 99

7.3 Optimisation of primary clarification (centrifuge or microfiltration) 109

7.4 Selection of membrane 122

7.5 Optimisation of operating conditions 124

7.6 Mechanism of flux decline 130

7.7 Ultrafiltration of primary clarified Stevia extract 141

7.8 Concentration by nanofiltration 155

8 Performance modelling of stevioside separation using membrane processing 161

8.1 Modelling of stirred ultrafiltration 162

8.2 Modelling of cross-flow ultrafiltration 174

8.3 Steady state 180

8.4 Transient state 180

9 Enhancement of stevioside recovery by diafiltration 195

9.1 Multiple stage diafiltration 197

10 Economics of the process 209

Index 215

A colour plate section falls between pages 12 and 13

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