Microarrays in Inflammation

, by Bosio, Andreas; Gerstmayer, Bernhard

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ISBN: 9783764383336 | 376438333X
Cover: Hardcover
Copyright: 12/1/2008

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The book is of interest to academic and industrial researchers but also clinicians who are working on inflammation related topics and would like to extend their knowledge about microarrays and the application thereof. After reading the book a researcher or clinician should be in the position to plan, perform and analyse or to critically review microarray experiments related to inflammation research.

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List of contributors	p. vii
Preface	p. xi
Microarray and inflammation: an introduction	p. 1
Methods and protocols for the generation of gene expression profiles in inflammation research
Sample preparation
Whole blood	p. 15
Separation of whole blood cells and its impact on gene expression	p. 31
Skin and skin models	p. 41
Adipose tissue	p. 51
Samples isolated by flow cytometry	p. 59
Magnetic cell sorting	p. 71
Single and rare cell analysis - amplification methods
T7 based amplification protocols	p. 81
Amplification of cDNA from single or rare cells by global PCR (exponential amplification)	p. 95
Selected applications of microarrays in inflammation research
Gene expression patterns in asthma	p. 107
Gene expression profiling in neurological and neuroinflammatory disorders	p. 115
Aspects of gene expression in B-cell lymphoma	p. 131
The genes behind rheumatology	p. 145
miRNA patterns in hematopoietic malignancies	p. 161
Cross-platform microarray analysis
Keep it simple: microarray cross-platform comparison without statistics	p. 179
A regulatory perspective
Microarrays in drug development: regulatory perspective	p. 199
Outlook
Complementary microarray technologies	p. 213
Index	p. 225
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Microarrays in Inflammation

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