Plant Virology Protocols

, by Foster, Gary D.; Johansen, I. Elisabeth; Hong, Yiguo; Nagy, Peter D.

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ISBN: 9781588298270 | 1588298272
Cover: Hardcover
Copyright: 4/1/2008

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Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section presents the most novel methods and step-by-step reproducible laboratory protocols to allow researchers more effective approaches to study plant viruses. This updated book will prove indispensable to laboratory investigators studying plant viruses.

Preface	p. v
Contributors	p. xiii
General Introduction
Plant-Virus Interactions	p. 3
Role of Capsid Proteins	p. 21
Role of Plant Virus Movement Proteins	p. 33
Multiple Roles of Viral Replication Proteins in Plant RNA Virus Replication	p. 55
Role of Silencing Suppressor Proteins	p. 69
Role of Vector-Transmission Proteins	p. 81
Viral Nucleic Acid Sequence/Function Analysis
In Vivo Analyses of Viral RNA Translation	p. 99
In Vitro Analysis of Translation Enhancers	p. 113
Identification of Plant Virus IRES	p. 125
Analysis of Geminivirus DNA Replication by 2-D Gel	p. 135
Begomoviruses: Molecular Cloning and Identification of Replication Origin	p. 145
Analysis of Viroid Replication	p. 167
Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs	p. 185
In Situ Detection of Plant Viruses and Virus-Specific Products	p. 201
Detection of siRNAs and miRNAs	p. 217
Cloning of Short Interfering RNAs from Virus-Infected Plants	p. 229
Solution Structure Probing of RNA Structures	p. 243
RNA Encapsidation Assay	p. 251
Protein Analysis and Investigation of Protein Function
Surface Plasmon Resonance Analysis of Interactions Between Replicase Proteins of Tomato Bushy Stunt Virus	p. 267
Biochemical Approaches for Characterizing RNA-Protein Complexes in Preparation for High Resolution Structure Analysis	p. 279
Probing Interactions Between Plant Virus Movement Proteins and Nucleic Acids	p. 293
Movement Profiles: A Tool for Quantitative Analysis of Cell-to-Cell Movement of Plant Viral Movement Proteins	p. 317
Analysis of siRNA-Suppressor of Gene Silencing Interactions	p. 331
Phosphorylation Analysis of Plant Viral Proteins	p. 339
Analysis of Interactions Between Viral Replicase Proteins and Plant Intracellular Membranes	p. 361
Membrane and Protein Dynamics in Virus-Infected Plant Cells	p. 377
Site-Directed Mutagenesis of Whole Viral Genomes	p. 395
Viral Protein-Nucleic Acid Interaction: South (North)-Western Blot	p. 405
Protein-Protein Interactions: The Yeast Two-Hybrid System	p. 421
NMR Analysis of Viral Protein Structures	p. 441
Localization of Viral Proteins in Plant Cells: Protein Tagging	p. 463
Microscopy/GFP/Protein Tagging/Infections Clones and Other Such Tools
Construction of Infectious Clones for RNA Viruses: TMV	p. 477
Construction of Infectious cDNA Clones for RNA Viruses: Turnip Crinkle Virus	p. 491
Construction of Infectious Clones for DNA Viruses: Mastreviruses	p. 503
Construction of Infectious Clones of Double-Stranded DNA Viruses of Plants Using Citrus Yellow Mosaic Virus as an Example	p. 525
Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones	p. 535
Analysis of Cell-to-Cell and Long-Distance Movement of Apple Latent Spherical Virus in Infected Plants Using Green, Cyan, and Yellow Fluorescent Proteins	p. 545
Agroinoculation: A Simple Procedure for Systemic Infection of Plants with Viruses	p. 555
Geminivirus: Biolistic Inoculation and Molecular Diagnosis	p. 563
Genomics, Host Factors and Plant Based Studies
Expression Microarrays in Plant-Virus Interaction	p. 583
Genome-Wide Screens for Identification of Host Factors in Viral Replication	p. 615
Phosphorylation of Movement Proteins by the Plasmodesmal-Associated Protein Kinase	p. 625
Virus-Induced Gene Silencing as a Tool to Identify Host Genes Affecting Viral Pathogenicity	p. 641
Yeast Two-Hybrid Assay to Identify Host-Virus Interactions	p. 649
Index	p. 673
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Plant Virology Protocols

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