Genetics Laboratory Investigations

For lab courses in genetics. THE definitive genetics lab manual and winner of 1998 Textbook Authors Association McGuffey Award in Life Sciences. Genetics Laboratory Investigations, 12/E is user-friendly for both faculty and studentshaving been developed by those actively involved in teaching general genetics. The manual stresses classical genetics, but includes some of the recent advances related to molecular and human genetics.

Preface

vii

Drosophila and Maize Experiments in Genetics: Monohybrid Crosses

1

(10)

Drosophila and Maize Experiments in Genetics: Dihybrid Crosses

11

(8)

Principles of Probability

19

(8)

The Chi-Square Test

27

(14)

Cell Reproduction: Mitosis

41

(10)

Meiosis in Animals: Oogenesis and Spermatogenesis

51

(8)

Meiosis in Angiosperms: Microsporogenesis and Megasporogenesis

59

(16)

Polytene Chromosomes from Drosophila Salivary Glands

75

(4)

Sex Chromosomes and Gene Transmission

79

(10)

The Sex Check: A Study of Sex Chromatin in Human Cells

89

(6)

Human Chromosomes

95

(26)

Linkage and Crossing Over

121

(20)

Genetics of Ascospore Color in Sordaria: An Investigation of Linkage and Crossing Over Using Tetrad Analysis

141

(8)

Open-Ended Experiments Using Drosophila: Locating a Mutant Gene in Its Chromosome

149

(6)

The Genetic Material: Isolation of DNA

155

(6)

Restriction Endonuclease Digestion and Gel Electrophoresis of DNA

161

(16)

Amplification of DNA Polymorphisms by Polymerase Chain Reaction (PCR) and DNA Fingerprinting

177

(16)

Transformation of E. coli

193

(8)

Gene Action: Synthesis of β-Galactosidase in Escherichia coli

201

(6)

Chromatographic Characterization of Drosophila melanogaster Mutants

207

(6)

Bacterial Mutagenesis

213

(6)

Gene Recombination in Phage

219

(8)

Polygenic Inheritance: Fingerprint Ridge Count

227

(10)

Population Genetics: The Hardy-Weinberg Principle

237

(12)

Population Genetics: The Effects of Selection and Genetic Drift

249

(8)

Applied Human Genetics

257

(16)

Supplemental Laboratory Topics

273

(5)

Plant Speciation by Interspecific Hybridization and Allotetraploidy

273

(2)

Chromosome Translocations in Rhoeo spathacea

275

(1)

Human Pedigree Analysis

276

(2)

Tagging and Amplification of DNA Using DNA Polymerase

278

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Genetics Laboratory Investigations

Summary

Table of Contents

Supplemental Materials